Antibody Discovery - Medical / Health Care - Pharmaceuticals
The antibody drug discovery process differs from the traditional drug discovery of small molecules as it requires the development of hybridomas and the engineering of antibodies to recognize a specific target protein. The production of recombinant antibodies in cancer treatment as well as the development of neutralizing antibodies for infectious diseases, has allowed antibody discovery platforms to interrogate the immune repertoire and develop novel antibody libraries using flow cytometry analysis and cell sorting. The WOLF cell sorter provides a simple, gently cell sorting solution for many workflows such as: Antibody Discovery from Single Bovine B-Cells, Isolate Antibody-Secreting CHO Cells, Recombinant protein production in CHO Cells.
Antibody Discovery
Due to the complexities with immunization, isolation of B-cells from blood, plating of viable single-cells, and sequencing of antibody antigen-binding regions, therapeutic antibody discovery is not without its challenges. The antibody-drug discovery process differs from the traditional drug discovery of small molecules as it requires the development of hybridomas and the engineering of antibodies to recognize a specific target protein.
The production of recombinant antibodies in cancer treatment as well as the development of neutralizing antibodies for infectious diseases, has allowed antibody discovery platforms to interrogate the immune repertoire and develop novel antibody libraries using flow cytometry analysis and cell sorting. The WOLF® cell sorter provides a simple cell sorting solution that gently sorts high antibody-producing clones and dispenses single-cells for optimal clone outgrowth.
WOLF Benefits
Efficient antibody screening by sorting into a 96- or 384-well plate.
Antibody drug discovery and the development of functional antibodies require sorting sensitive cells into multi-well plates for clonal outgrowth. A simple and gentle cell sorting solution in the lab is key to an efficient antibody screening process.
Eliminate carryover and instrument contamination through disposable microfluidic cartridges.
The simplest way to ensure no cross-contamination between cell lines is to replace the entire system fluidics at once with a new disposable cartridge. No instrument clean-up required.
Gentle cell sorting leads to high clonal outgrowth.
Compared to traditional droplet-based cell sorters running under high-pressure, the WOLF provides an alternative sorting method where cells do not undergo a decompression shock before being deposited in the well.
Simple sorting workflow improves user efficiency.
Even for flow cytometry beginners, the WOLF has an intuitive and basic software workflow. It takes a day of training before novice users are up and running in their own lab.
Compact, benchtop cell sorting in the lab.
Many laboratories do not have space for a large, conventional cell sorter. Save on space by implementing the WOLF into a cell line development workflow, at only 2 cubic feet. The compact design fits in biosafety cabinet for sterile cell sorting.
As fast as 9 minutes for a 96-well plate and 33 minutes for a 384-well plate.
The WOLF instrument setup is rapid and automated. When followed by a gentle sort and no lengthy instrument clean up, the overall process is simple and straightforward.
Antibody Discovery from Single Bovine B-Cells
Bovine immunoglobulins have great potential for development as clinical treatments and research tools targeting a broad variety of antigens. Bovine antibody structures slightly differ from their human equivalent and provide opportunities to identify antibodies with unique specificities and obtain the antibody sequence of their light chains. When compared with the BD FACSAria, sorting B cells with the WOLF cell sorter resulted in twice as many single-cells with detectable OneStep RT-PCR product, and 41% more single-cells were sequenced for both heavy and light chains. The WOLF cell sorter optimizes the selection process of antibody candidates and speeds up the recombinant antibody development all the way to antibody production.