Cell Line Development - Medical / Health Care
Generating cell lines has often been inefficient and affected by inaccurate limited dilution methods or the shear stress caused by conventional flow cytometer and traditional high-pressure cell sorters. NanoCellect Biomedical provides cell sorting solutions that allow to sort cells and establish stable cell lines using gentle low-pressure microfluidics. The WOLF’s ability to protect cell viability after sorting makes it an integral part of any cell line development workflow such as: Recombinant protein production in CHO Cells, Sorting stem cell cultures, Antibody discovery.
Cell Line Development
The development of cell lines following a stable transfection step for the purpose of gene expression or recombinant protein production is an important method used by researchers. Generating cell lines has often been inefficient and affected by inaccurate limited dilution methods or the shear stress caused by conventional flow cytometer and traditional high-pressure cell sorters. NanoCellect Biomedical provides cell sorting solutions that allow us to sort cells and establish stable cell lines using gentle low-pressure microfluidics.
This protects cell viability after sorting and improves sorting efficiency by increasing the number of viable clones growing per plate. Contaminate and biohazard–free, the WOLF cell sorter’s rapidly exchangeable cartridge and tubing set eliminates carryover between samples and allows for quick and easy cleanup. More than a cell printer, the WOLF is a FACS (fluorescence-activated cell sorting) instrument capable of sorting based on 5 optical parameters that provide well-by-well index cellular data.
- Efficient antibody screening by sorting into a 96- or 384-well plate.
Developing cell lines requires to sort sensitive cells. The ability to sort cells in the lab when they are ready ensures an efficient process to improve cell viability and clonal outgrowth.
- Eliminate carryover and instrument contamination through disposable microfluidic cartridges.
The simplest way to ensure no cross-contamination between cell lines is to replace the entire system fluidics at once with a new disposable cartridge. No instrument clean-up required.
- Gentle cell sorting leads to high clonal outgrowth.
Compared to traditional droplet-based cell sorters running under high-pressure, the WOLF provides an alternative sorting method where cells do not undergo a decompression shock before being deposited in the well.
- Simple sorting workflow improves user efficiency.
Even for flow cytometry beginners, the WOLF has an intuitive and basic software workflow. It takes a day of training before novice users are up and running in their own lab.
- Compact, benchtop cell sorting in the lab.
Many laboratories do not have space for a large, conventional cell sorter. Save on space by implementing the WOLF into a cell line development workflow, at only 2 cubic feet. The compact design fits in a biosafety cabinet for sterile cell sorting.
- As fast as 9 minutes for a 96-well plate and 33 minutes for a 384-well plate.
The WOLF instrument setup is rapid and automated. When followed by a gentle sort and no lengthy instrument clean up, the overall process is simple and straightforward.
N1 Single-Cell Dispenser
The N1 Single-Cell Dispenser is an add-on accessory for the WOLF® cell sorter used for single-cell deposition of viable cells into 96- or 384- well plates. Typically, wells are pre-filled with cell culture media, while the same media is used as the sheath buffer to ensure cell viability. Each well can receive anywhere from 1 to 100 cells and the whole plate can be processed as fast as 9 min and 33 min for 96- or 384- well plates, respectively. The pairing of the low-pressure WOLF® cell sorter and the N1 single-cell dispenser greatly improves cell integrity from that of conventional sorters, providing higher populations of viable and healthy clones, therefore facilitating stable cell line generation.