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6 applications found
Cells were grown in standard DMEM basal media containing penicillin, streptomycin, and GlutaMAX™ with 10% (v/v) FBS or 10% SimPL-Serum in 6-well plates. Cells were grown in suspension culture and subcultivated every 3 days at an average cell density of 0.4 x 10^5 cells/mL. ...
ByAgulos Biotech, LLC based in Lake Mills, WISCONSIN (USA)
Media supplemented with SimPL also contained additional magnesium sulfate sufficient to achieve a final media concentration of 5.7 mM Mg. 3T3-L1 cells were passaged using standard techniques in each condition 10 times before seeding a 96-well plate for label-free cell counting with a BioSpa 8 automated incubator and Cytation 5 multi-mode reader. Data represent counts of cells present within imaged well center. Comparable performance was seen throughout all prior ...
ByAgulos Biotech, LLC based in Lake Mills, WISCONSIN (USA)
Prior to growth evaluation, cells were passaged using standard techniques in 10% FBS with standard DMEM/F12 basal media and were seeded into each condition without adaptation into a 96-well plate. ...
ByAgulos Biotech, LLC based in Lake Mills, WISCONSIN (USA)
Prior to growth evaluation, cells were passaged using standard techniques in 10% FBS with standard DMEM/F12 basal media and were seeded into each condition without adaptation into a 96-well plate. Data represent cell counts relative to the initial seeding density average of each condition obtained from label-free cell counting with a BioSpa 8 automated incubator and Cytation 5 multi-mode ...
ByAgulos Biotech, LLC based in Lake Mills, WISCONSIN (USA)
FluidFM® for Virology - Get a unique control over your single virion-single cell in-vitro experiment! Typically, viral infections are studied in bulk experiments where many cells in culture are exposed to the virus simultaneously, making it difficult to study single virus entry events or the spread of the viral infection between individual cells. With the gentle, micro-channeled, and force feedback-controlled FluidFM Probes, single virions can be deposited onto selected cells allowing unprecedented control to ...
ByCytosurge AG based in Glattbrugg, SWITZERLAND
Life science is a sprawling field of study concerned with all living organisms and some non-cellular entities that can dramatically impact life as we know it. Research in life science spaces concerns an impossibly varied range of organic structures from sub-microscopic infectious agents to entire ecosystems in a truly cross-disciplinary research effort to better understand and preserve life on our planet. ...
ByMIPAR Image Analysis based in Worthington, OHIO (USA)