DNA-protein crosslinks induced by formaldehyde and its repair process

KCL-SDS assay was used to explore DNA-Protein Crosslinks (DPC) effect induced by formaldehyde (FA) and the repair. Gaseous FA could cause DPC at concentrations as 1.0 mg m−3 and 3.0 mg m−3 (p < 0.01), which was removed within 12 h. Meanwhile, in vitro studies showed that 75 µmol L−1 liquid FA could significantly induce DPC in HepG2 cells, and was removed after 18 h and 24 h. These results suggested that FA could induce DPC at relatively high concentrations, and removing of DPC in vivo required shorter time than that in vitro.

Keywords: formaldehyde, in vivo, in vitro, DPC, DNA-protein crosslinks, repair, DNA, proteins