Increasing Cell Adherence with a Hypoxia Workstation

Abstract
A major criticism of all-in-one workstations (sometimes known as incubated workstations or incubated glove boxes) over using traditional incubators and biosafety cabinets for cell culture is that the exposure to excessive vibration will affect cell growth and adhesion. Here we tested the cells’ capabilities to attach in a Baker Ruskinn SCI-tive hypoxia workstation as compared to a standard CO2  incubator with and without rubber feet (or ferrules) attached to the interior floor tray. The floor tray with all 15 ferrules did remarkably better than a standard incubator, increasing cell adhesion by 25% over a standard incubator. This was confirmed by measuring the reduction in vibration in each planar direction. The floor tray with ferrules significantly reduces vibration exposure to the cells and increases adhesion, both critical for cell growth.