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Antibiotic Resistance And Virulence Genes Articles & Analysis
15 articles found
Fosmid libraries represent a pivotal advancement in the field of genomics and molecular biology, offering a robust method for genomic cloning and analysis. These libraries are particularly valuable due to their ability to retain large fragments of DNA, making them essential for various applications, including sequencing and functional studies. Understanding Fosmids Fosmids are vector systems ...
Stable cell lines are an indispensable tool in the realm of biomedical research, playing a crucial role in advancing our understanding of cellular processes and disease mechanisms. Unlike primary cells that have a limited lifespan, stable cell lines offer a consistent and renewable source of cells, making them invaluable for long-term studies and industrial applications. This article explores the ...
Meanwhile, dry lab bioinformatics analysis encompasses tasks like data quality control, the elimination of human sequences, comparison of microbial species sequences, and analysis of drug resistance or virulence genes. Unveiling the Process: Key Stages Sample Collection: Precision in collecting samples from the primary infection site augments ...
Microbes are present almost everywhere. They play a vital role in cycling carbon, release important compounds, and may be associated with infectious diseases. Metagenomics is the culture-independent genomic research of microbial communities. Metagenomics presents a powerful tool to study prokaryotes and viruses in the environment via the analysis of their DNA obtained directly from environmental ...
Microbes are present almost everywhere. They play a vital role in cycling carbon, release important compounds, and may be associated with infectious diseases. Metagenomics is the culture-independent genomic research of microbial communities. Metagenomics presents a powerful tool to study prokaryotes and viruses in the environment via the analysis of their DNA obtained directly from environmental ...
The figure below summarizes the application of Capture-Seq in pathogen detection, identification, characterization, and determination of virulence/resistance determinants (source: Gaudin and Desnues, 2018). For pathogen detection, this technology is able to detect even low-copy number pathogens and even detect different pathogens at the same time. ...
Large amounts of antibiotic resistance genes (ARGs) have been found throughout estuaries in coastal China, and scientists say they’re causing potentially dangerous changes to local bacteria. When bacteria mutate and become resistant to antibiotics, it endangers human health and life. If antibiotics can’t work properly, infectious diseases will increase, and health-care costs will ...
Whereas the hygienic condition of drinking and bathing water by law must be monitored by culture-based methods, for quantification of microbes and antibiotic resistance in soil or the aquatic environment, often molecular genetic assays are used. For comparison of both methods, knowledge of their correlation is necessary. Therefore the population of total bacteria, Escherichia coli, ...
Antibiotic resistant bacteria are an emerging global problem which threatens to undermine important advances in modern medicine. The environment is likely to play an important role in dissemination of antibiotic resistance genes (ARGs) among both environmental and pathogenic bacteria. Wastewater treatment plants (WWTPs) accumulate both chemical and biological waste from the surrounding urban ...
The occurrence of antibiotic resistance genes (ARGs) and resistant bacteria was quantified in 17 water samples collected across Bosten Lake, Xinjiang, China. The heterotrophic plate count method was used to detect the levels of sulfonamide- and tetracycline-resistant bacteria, which have mean concentrations of 2.50 × 105 and 4.63 × 103 CFU/mL, respectively. The resistance genes of ...
Since lysogenic phages can act as vectors for antibiotic-resistance and virulence factors, the prevalence of the mentioned genes, as well as that of an integrase-encoding gene (int) specific for Enterococcus faecalis phages was determined. Up to 60 and 54% of the bacterial fractions and enterococci, ...
A key problem challenging public health officials' efforts to stem the spread of antibiotic resistance is the potential increase of resistance in the environment. Yet, despite recent and significant changes to agricultural land in New Zealand, as well as the sector's high antibiotic use, the influence on antibiotic resistance in the environment remained uncharacterised. Spatial ...
The purpose of this study was to investigate the occurrence of antibiotic resistance genes (ARGs) in water used for irrigation in the Werribee River Basin, Australia, including river water and reclaimed effluent water (reclaimed water). Samples of reclaimed water, collected over a one-year period, were screened for the occurrence of ARGs using PCR detection assays. The presence of ARGs in the ...
This study reports the development of several cloning vectors for bifidobacteria based on the replicon of pBC1, a cryptic plasmid from Bifidobacterium catenulatum L48 thought to replicate via the theta mode. These vectors, in which antibiotic resistance genes encoding either erythromycin or tetracycline resistance acted as selection markers, were able to replicate in a series of eight ...
Transfer of class 1 integron-mediated antibiotic resistance genes has been demonstrated under laboratory conditions. However, there is no information concerning the transfer of these genes in an agricultural environment. The present study sought to determine if integron-mediated streptomycin and sulfisoxazole resistance genes could be transferred from Shiga toxin-producing Escherichia coli (STEC) ...