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Antibiotic Resistance Genes Articles & Analysis
15 articles found
The structure of a fosmid consists of several key components: an origin of replication, a selectable marker (usually an antibiotic resistance gene), and cloned DNA fragments. The combination of these elements allows for the maintenance and propagation of large DNA inserts within host cells. ...
This consistency is achieved through the integration of a specific gene of interest into the host cell’s genome, allowing it to be perpetually expressed. Researchers often select for stable cell lines by integrating antibiotic resistance genes alongside target genes, enabling them to isolate and cultivate ...
Meanwhile, dry lab bioinformatics analysis encompasses tasks like data quality control, the elimination of human sequences, comparison of microbial species sequences, and analysis of drug resistance or virulence genes. Unveiling the Process: Key Stages Sample Collection: Precision in collecting samples from the primary infection site augments detection rates ...
The coupling of this method with function-based activity is a powerful technique for the discovery of new functional genes from uncultured microbes. Biotechnological applications Currently, there is a global political drive to promote white biotechnology as a key feature of modern industrialized society. ...
The coupling of this method with function-based activity is a powerful technique for the discovery of new functional genes in uncultured microbes. Biotechnological applications Currently, there is a global political drive to promote biotechnology as a key feature of modern industrialized society. ...
After amplification, NGS is performed to determine the sequence of the target genes and bioinformatic analysis follows to identify the sequences. Capture sequencing is also commonly used in pathogen detection and classification. ...
Large amounts of antibiotic resistance genes (ARGs) have been found throughout estuaries in coastal China, and scientists say they’re causing potentially dangerous changes to local bacteria. ...
Whereas the hygienic condition of drinking and bathing water by law must be monitored by culture-based methods, for quantification of microbes and antibiotic resistance in soil or the aquatic environment, often molecular genetic assays are used. ...
Antibiotic resistant bacteria are an emerging global problem which threatens to undermine important advances in modern medicine. The environment is likely to play an important role in dissemination of antibiotic resistance genes (ARGs) among both environmental and pathogenic bacteria. Wastewater treatment ...
The occurrence of antibiotic resistance genes (ARGs) and resistant bacteria was quantified in 17 water samples collected across Bosten Lake, Xinjiang, China. ...
Since lysogenic phages can act as vectors for antibiotic-resistance and virulence factors, the prevalence of the mentioned genes, as well as that of an integrase-encoding gene (int) specific for Enterococcus faecalis phages was determined. Up to 60 and 54% of the bacterial fractions and enterococci, respectively, harbored ...
Spatial and temporal dynamics of antibiotic resistance genes in freshwater biofilms from NZ's fourth longest river as it transitioned between low and high intensity farming were examined for 1 year. Polymerase chain reaction was employed to gauge the level of resistance present. Biofilms were screened for 10 ...
The purpose of this study was to investigate the occurrence of antibiotic resistance genes (ARGs) in water used for irrigation in the Werribee River Basin, Australia, including river water and reclaimed effluent water (reclaimed water). ...
This study reports the development of several cloning vectors for bifidobacteria based on the replicon of pBC1, a cryptic plasmid from Bifidobacterium catenulatum L48 thought to replicate via the theta mode. These vectors, in which antibiotic resistance genes encoding either erythromycin or tetracycline resistance acted as ...
Transfer of class 1 integron-mediated antibiotic resistance genes has been demonstrated under laboratory conditions. ...