blood sampling Articles
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Dependence of T-lymphocyte activation on the extent of cellular damage
Mechanically damaged cells release intracellular substances, resulting in the activation of CD4- and CD8-positive T-lymphocytes. The goal of this investigation was to quantify and compare fractions of activated CD4- and CD8-positive T-lymphocytes, based on the quantity of damaged cells in a given blood sample. Blood samples were mechanically stressed by vortexing intensely. Subsequently, ...
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Chromosomal aberrations induced by a low dose of neutron irradiation and hyperthermia
The purpose of this study was to investigate the effect of Hyperthermia (HT) on the Frequency of Chromosomal Aberrations (FCA) induced by a low dose of neutron irradiation. Blood samples were exposed to HT, 6cGy neutrons, HT + neutrons and neutrons + HT, and the FCA was scored in metaphase. HT alone at 41.5°C had no effect on FCA; however, the FCA was significantly higher at 43°C (p Keywords: low ...
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What is the reliability of chromosomal aberration assays as biomarkers of individual sensitivity towards ionising radiation?
The development of biomarkers of susceptibility or sensitivity towards ionising radiation is important for the identification of individuals who may be at increased risk for the development of cancer after occupational, environmental or medical exposures. In this study we investigated the inter- and intra-individual variation of the MN assay and the G2 assay in irradiated lymphocytes to assess ...
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Chromosomal aberrations in radiation workers of radiology departments in Northern Iran-Babol
We determined the frequency of chromosomal aberrations using the metaphase analysis method in radiation workers of radiology departments in Babol. Blood samples were prepared from 62 radiology workers and 29 individuals as controls, and lymphocytes were cultured in a culture medium. Results showed that the mean frequencies of the total chromosomal aberration in radiation workers are significantly ...
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Glucose biosensor based on platinum nanowires: a clinical study
We describe the process of modification of the platinum (Pt) nanowire surface and subsequent immobilisation of enzyme glucose oxidase (GOx) on Pt nanowires (PtNWs) for direct glucose measurement. A cyclic voltammetry technique was used to measure electrochemical oxidation between GOx and glucose. PtNW arrays were first fabricated and then functionalised with cysteamine, which in turn acted as a ...
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Transcriptional expression of H2B, CTP synthase and PLK3 genes in whole blood exposed to 60Co gamma radiation
Ionising radiation induces complex molecular responses in human cells resulting in changes at mRNA and protein expression. Limited data is available on the transcriptional status of functional genes in response to ionising radiation using peripheral blood mononuclear cells (PBMCs). In the present study, transcriptional profiles of Histone 2B, CTP synthase and PLK3 were studied. Blood samples were ...
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Development of an in vivo assay for detection of non-targeted radiation effects
An adaptive response may be defined as the effect of a small priming dose of radiation modifying the anticipated cellular response of the same tissues so as to alter the predicted response to a larger dose of radiation. We and many others have demonstrated that at low radiation doses (less than 0.5 Gy) the lethal and mutational effect of the radiation is mainly, possibly entirely, due to the ...
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Analysis of low-angle X-ray scattering profiles from irradiated blood samples
The Transfusion-Associated Graft-Versus-Host Disease (TA-GVHD) is a serious potential complication of blood transfusions that is essentially prevented by blood product irradiation. In this study, scattering profiles from lyophilised and irradiated samples of plasma (PL) and formed elements (MC) were compared with non-irradiated ones. Fresh blood specimens were obtained from volunteers by vein ...
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The monocyte dysfunction induced by acute tetramine poisoning and corrected by continuous blood purification
The monocyte function of patients with severe tetramine poisoning and the effects of sequential hemoperfusion (HP) and continuous veno-venous hemofiltration (CVVH) on the immune status of the patients were investigated. Eleven patients with severe acute tetramine poisoning were treated with sequential HP and CVVH. The APACHE II score and Glasgow score were used to evaluate the disease status ...
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Low doses of γ–radiation induce consistent protein expression changes in human leukocytes
Twenty percent of cancer patients experience adverse effects after radiotherapy. The therapeutic doses are adjusted to the most sensitive individuals, resulting in a suboptimal dose for many patients. At present there is no screening system available to predict individual radiosensitivity. The main aim of this study is to investigate differences in protein expression pathways induced by low doses ...
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Transcriptional expression of H2B, CTP synthase and PLK3 genes in whole blood exposed to 60Co gamma radiation
Ionising radiation induces complex molecular responses in human cells resulting in changes at mRNA and protein expression. Limited data is available on the transcriptional status of functional genes in response to ionising radiation using peripheral blood mononuclear cells (PBMCs). In the present study, transcriptional profiles of Histone 2B, CTP synthase and PLK3 were studied. Blood samples were ...
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PKU and MSUD screening for phenylketonuria and maple syrup urine disease
Phenylketonuria (PKU) and Maple syrup urine disease (MSUD), branched-chain ketoaciduria are genetically transmitted metabolic diseases. PKU results in elevated Phenylalanine levels due to deficient activity of the enzyme Phenylalanine hydroxylase. In the MSUD state, elevated levels of leucine (Leu), isoleucine (Ile), valine (Val), and alloiso-leucine can be detected in blood. The increased ...
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Impact of feeding flaxseed oil on delaying the development of osteoporosis in ovariectomised diabetic rats
This work is aimed to study the effect of diabetes on bone health and to evaluate the impact of feeding flaxseed oil on delaying osteoporosis. About 70 female albino rats were included; 30 were ovariectomised (ovx). Experimental diabetes was induced. Rats were classified in groups as control, sham, diabetic, diabetic received flaxseed oil in the diet, ovx, ovx-diabetic and ovx-diabetic received ...
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Clinical Research On-Demand: How the University of Washington’s Theberge Lab is Using Tasso to Study Unpredictable Events in Real Time - Case Study
Many important research questions cannot be studied under controlled circumstances. Events like natural disasters or disease exposure often occur without warning and prevent in-person clinic visits, making timely observation difficult without a flexible approach. At the University of Washington, researchers are partnering with Tasso to study these scenarios by deploying on-demand remote ...
By Tasso, Inc.
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Blood alcohol content analysis using nitrogen carrier gas
Results are used in court to provide quantitive levels of BAC, which makes it one of the most commonly practised analyses in forensic laboratories. The large number of samples and requirement for speed of sample processing mean that analysis needs to be conducted quickly, whilst giving reliable and accurate results. Alcohol consumption can seriously affect the ability of a driver to operate ...
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Radiation-induced DNA damage in patients exposed to X-rays during cerebral angiography
Population exposure to diagnostic and therapeutic ionising radiation is likely to increase worldwide, and this has raised concerns about the stochastic effects of low doses of radiation exposure. The present study was aimed to study the incidence of Chromosomal Aberrations (CAs) in patients exposed to low doses of X-rays during diagnostic interventional radiological procedures. Peripheral blood ...
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Bio-Me part of unique Nordic IBD initiative
Bio-Me is one of the participants in a new Nordic project that will develop personalized treatment for IBD patients. In December 2018, the Nordic Council’s research department awarded Nordfors 30 million for a research project with the theme of personalized medicine for inflammatory bowel disease (IBD). The study has been titled NORDTREAT – The Nordic IBD treatment strategy ...
By Bio-Me
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Universal Diagnostics Initiates Observational Study in the US to Evaluate Cell Free DNA Blood Test for Adenomas and Colorectal Cancer
SEVILLE, Spain--(BUSINESS WIRE)--Universal Diagnostics (UDX), an in-vitro diagnostics company developing minimally-invasive, blood-based solutions for detecting cancer early, announced today that it has started a prospective, multi-center observational study in the US for its investigational advanced adenoma (precursor lesions) and colorectal cancer (CRC) detection blood test. UDX is performing ...
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Israeli hospital pilots rapid diagnostics of blood cancer with Sight Diagnostics
Israeli-founded firm Sight Diagnostics, the company that uses AI and cutting-edge hardware to deliver rapid, complete blood count results, has signed a new research agreement with Jerusalem’s Shaare Zedek Medical Center to identify the possibility of blood cancer using a combination of images and data collected from Sight’s OLO device and patient clinical information provided by the ...
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Bio-tech firm recruiting Yukon men for trial, Prostate cancer group says it could save lives
The Yukon chapter of Ride For Dad says a new blood test developed by an Alberta-based company could save the lives of men at risk of prostate cancer. (iStock/Getty Images) A bio-technology company based in Alberta is testing a new process that could result in fewer Yukon men travelling out of territory for prostate cancer biopsies. Private research company Nanostics Inc. and the University of ...
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