Cdna Library Construction Articles & Analysis: Older
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Similar to the m6A and m1A detection, m5C RNA immunoprecipitation (m5C-RIP) enriches fragmented RNA with m5C-specific antibodies prior to cDNA library construction and sequencing, whereas BS-seq does not allow differentiation between hm5C and m5C, revealing only the presence of cytosine modifications in RNA transcripts. ...
The workflow of single-cell RNA-seq generally involves the following steps: single-cell isolation, cDNA library construction, RNA-seq, and bioinformatics. Applications of single-cell RNA-seq： Stem cell differentiation Embryogenesis Whole-tissue analysis Single-cell RNA-seq for whole-organism studies Disease biology and ...