culture medium Articles
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Fibrinogen induces biofilm formation by streptococcus suis and enhances its antibiotic resistance
In this study, we showed that supplementing the culture medium with fibrinogen induced biofilm formation by Streptococcus suis in a dose-dependent manner. Biofilm-grown S. suis cells were much more resistant to penicillin G than planktonic cells. S. suis bound fibrinogen to its surface, a property that likely contributes to biofilm ...
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Analysis of a novel insect cell culture medium-based growth medium for bartonella species
Human- and animal-pathogenic Bartonella species are fastidious and slow-growing bacteria difficult to isolate and cultivate. We describe a novel, easy-to-prepare liquid medium for the fast and reliable growth of several Bartonella spp. that does not affect bacterial protein expression patterns or interactions with host ...
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Construction and Culture Protocol of Neural Stem Cells Protocol
Because neural stem cells (NSCs) have the potential of self-renewal and multidirectional differentiation, the method of suspended neural bulb culture can be used to obtain and study. After processing the embryonic brain tissue into a single cell, only the cells with the ability of self-renewal can be cloned and proliferated into a suspended nerve ball in the culture medium, and with the progress ...
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Module 3: Culture Medium
This is the third module in a four part series titled “Zero to One on the basic science behind cultivated meat”. You can click on these links to access: Course Overview, Module 1 (Cell Culture Basics), Module 2 (Cell Sources), Module 4 (Process monitoring). Learning objectives By the end of this module, you should understand: Basal culture medium composition and sources; ...
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The creative potential of medium-sized and small Greek cities: reflections on contemporary cultural strategies
As the creative city discourse has come to the centre of cultural policy debates, its rhetoric often neglects smaller cities and emphasises the cases of large cities that employ culture as a driver for economic growth. However, the cultural strategies of many medium sized and small cities focus more on social objectives. This seems to be the case for Greek cities of medium and small size. In ...
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Chromosomal aberrations in radiation workers of radiology departments in Northern Iran-Babol
We determined the frequency of chromosomal aberrations using the metaphase analysis method in radiation workers of radiology departments in Babol. Blood samples were prepared from 62 radiology workers and 29 individuals as controls, and lymphocytes were cultured in a culture medium. Results showed that the mean frequencies of the total chromosomal aberration in radiation workers are significantly ...
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Effects on CD34+ haematopoietic stem cells induced by low doses of alpha irradiation
To study the normal cell response to low doses of radiation we have irradiated CD34+ haematopoietic stem cells with alpha particles with doses between 0.3 and 0.6 Gy. Radiation sources used are 222Rn and his daughters 218Po and 214Po previously dissolved in culture medium. CD34+ cells are exposed to alpha radiation and cultured for different times and doses, and later, survival, apoptosis ...
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Protective effect of curcumin on γ-radiation-induced sister chromatid exchanges in human blood lymphocytes
The present work evaluates the radioprotective effect of curcumin on γ-radiation-induced genetic toxicity. The DNA damage was analysed by the frequencies of Sister Chromatid Exchanges (SCEs). Human lymphocytes were treated in vitro with 5.0 γg/ml of curcumin for 30 min at 37°C then exposed to 1, 2 and 4 Gy γ-radiation. The lymphocytes were cultured in (RPMI) 1640 tissue culture medium and ...
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Entrepreneurship in Lebanon: a model for successes and failures
Entrepreneurs face different types of difficulties and operate in an unstable environment characterised by regional political chaos and local financial, legal, infrastructural, educational and cultural impediments. Small and Medium-sized Enterprises (SMEs) are the heart of any country; they empower its economy, heighten its competitive position, alleviate its employment problems and, in general, ...
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Establishment of a Stable Organoid Culture System: Customization and Standardization
Prefix Organoids are miniature organs created in vitro from adult stem cells (ASCs) or pluripotent stem cells (PSCs). They form three-dimensional (3D) tissue-like structures through the self-organization, self-renewal, and differentiation capabilities of stem cells. Compared to traditional two-dimensional (2D) cell cultures or spheroids, organoids closely resemble living organs in terms of ...
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Development of an in vivo assay for detection of non-targeted radiation effects
An adaptive response may be defined as the effect of a small priming dose of radiation modifying the anticipated cellular response of the same tissues so as to alter the predicted response to a larger dose of radiation. We and many others have demonstrated that at low radiation doses (less than 0.5 Gy) the lethal and mutational effect of the radiation is mainly, possibly entirely, due to the ...
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A Brief Introduction to Protease
Protease is a general term for a class of enzymes that hydrolyze protein peptide chains. According to the way they degrade polypeptides, they are divided into two categories: endopeptidases and telopeptidases. Endopeptidase can cut the large molecular weight polypeptide chain from the middle to form the smaller molecular weight prions and peptones. Telopeptidases can be further divided into ...
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Mechanism of Action of Paclitaxel
Paclitaxel is a natural anticancer drug with the molecular formula C47H51NO14. It has been widely used in the clinical treatment of breast cancer, ovarian cancer, and certain head and neck cancers, as well as lung cancer. Its novel and complex chemical structure, extensive and significant biological activity, unique mechanism of action, and scarce natural resources have gained immense favor among ...
By BOC Sciences
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Label-based Protein Quantification Technology: iTRAQ, TMT, SILAC
Changes in the number of proteomes in a cell have a big effect on many aspects of life. For example, the occurrence and development of many diseases are often accompanied by abnormal expression of certain proteins. Quantitative proteomics is the accurate quantification and identification of all proteins expressed in a genome or all proteins in a complex mixed system. At present, quantitative ...
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GMP Grade Cytokine Needed for Cell Therapy Manufacturing
GMP growth factors and cytokines designed for therapeutic manufacturing are essential components of defined media. To date, the cell therapy industry has accepted GMP terms for this reagent class, despite the fact that there is no direct oversight by regulators. These proteins are intended for use in further manufacturing processes and do not come into direct contact with patients. In fact, cell ...
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Rapid Development of the Industrial Enzyme Industry Meets Various Production Needs
Industrial enzymes refer to biological products with catalytic function prepared after enzymes are processed. There are more than 3,000 kinds of enzymes found in the world, of which less than 100 kinds of enzymes can be produced on a large scale. Industrial enzyme preparations have the advantages of high efficiency, low energy consumption, good specificity, and mild reaction conditions. They are ...
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What are Host Cell Proteins and Its Effect on Biopharmaceutical Development
Host cell proteins (HCPs) are process-related impurities produced by host cells and are typically present at low levels in recombinant biopharmaceutical products. Enzyme-linked immunosorbent assay (ELISA) has traditionally been used to monitor the total content of HCP in the production of therapeutic protein drugs. Now, with the continuous improvement and upgrading of technology, liquid ...
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Pooled Human Cryopreserved Hepatocytes
Hepatocytes are the chief functional cells of the liver, responsible for the selective uptake, metabolism and excretion of most drugs. Cells for use in plate cultures or suspension assays represent a key tool for predicting properties such as hepatotoxicity, metabolism, and potential drug-drug interactions. Since these cells mimic the properties of the liver, researchers can evaluate the ...
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Vaginal lactobacilli inhibit growth and hyphae formation of Candida albicans
Abstract Lactobacillusspecies are the predominant vaginal microbiota found in healthy women of reproductive age and help to prevent pathogen infection by producing lactic acid, H2O2 and anti-microbial compounds. Identification of novel vaginalLactobacillus isolates that exhibit efficient colonisation and secrete anti-Candida factors is a promising strategy to prevent vulvovaginal candidiasis. ...
By KoBioLabs
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Cultured Protein: A Guide to Every Cell-Based Meat Startup in the World Right Now
The alternative protein market is on fire right now, estimated to be worth approximately $2.2Bn, and cultivated meat is expected to play an important role in the shift from traditional meat products. Experienced scientists are engaged in cellular agriculture with the aim of reducing animal-based agriculture’s negative impact on both the planet and the suffering of animals and at the same ...
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