Cytometer Articles & Analysis
6 articles found
For example, the most advanced mass spectrometry flow cytometer can use a variety of metal element probes. FC analysis can characterize individual cells in terms of cell surface or internal antigen expression, subcellular components (such as mitochondria), and cell dynamic properties (such as changes in protein expression, calcium flow, membrane potential, and cell ...
Cells were analyzed using the BD FACSCanto II flow cytometer (Becton Dickinson, Franklin Lakes, New Jersey, USA) and the FlowJo software (Becton Dickinson, Franklin Lakes, New Jersey, USA). 2.10 Immunofluorescence analysis Caco-2 cells were seeded on glass cover slips in 24-well plates in IMDM medium containing 10% FBS and 1% penicillin/streptomycin and grown for 24 h at ...
Nano-flow cytometry is a revolutionary technology that has the potential to transform early disease detection and diagnosis. Nano-flow cytometers are able to detect and analyze individual nanoparticles, including extracellular vesicles and viruses, with high sensitivity and accuracy. ...
The CD4+ CD25+ Foxp3+ Treg population was analyzed using a BD FACSVerse™ Flow Cytometer (BD Bioscience). Intestinal microbiota analyses Intestinal microbiota was observed as described previously.46 Briefly, the V3-V4 region of the 16S rRNA gene in total genomic DNA collected from cecum was amplified using the barcoded primers 341F and 805R. ...
The CD4+CD25+Foxp3+ T cell population was counted using a BD FACSVerse™ Flow Cytometer (BD Biosciences). Cecum Microbiota Analysis Cecum microbiota were analyzed as previously described with minor modifications (Lim et al., 2016; Jang et al., 2019; Kim et al., 2019). ...
The new Flow Cytometry functional assay introduced, Emosis HIT Confirm®, overwhelms these limitations, and allows using this functional assay, on demand, in any site equipped with a flow cytometer. Only a platelet rich plasma from a single healthy donor is required, without any need for a cumbersome donor selection, and multiple testing. ...