Expressed Proteins Articles & Analysis
80 articles found
What is Protein Acetylation? At its core, Protein acetylation is the addition of an acetyl group (—COCH₃) from acetyl-coenzyme A (acetyl-CoA) to a protein. ...
Structure and Formation VLPs are typically composed of viral proteins that self-assemble into spherical or filamentous structures resembling native viruses. ...
Through its impact on translation efficiency and mitochondrial function tRNA methylation modification also promotes tumor cell proliferation by controlling cell cycle-related protein expression. Cell cycle progression within cells is a critical biological function whose stoppage and progression rely entirely on cell cycle-related protein ...
Recombinant protein expression is a cornerstone of modern biopharmaceutical development, providing the means to produce proteins with therapeutic potential, enzymes, and antibodies. ...
On the other hand, IHC is focused on identifying specific proteins within a tissue sample using antibodies. By employing various tags or enzyme-linked systems, IHC enables the visualization of protein expression patterns. ...
Immunohistochemistry: Frozen sections enable the study of protein expression within lung tissues. Specific antibodies can be used to target and visualize proteins associated with inflammatory responses, fibrosis, and cellular proliferation. Molecular Studies: Techniques such as in situ hybridization can be performed on frozen sections to ...
They possess a high transfection efficiency, making them ideal for gene expression studies, protein production, and functional assays. The use of HEK293T as the background for the Monkey DLL3 stable cell line enhances the ability to manipulate the genetic material effectively, enabling the expression of DLL3 in a controlled manner. ...
The principle is to clone one or several antigen genes of a pathogen into host cells, causing them to express the corresponding antigen protein. These proteins are then extracted for use in the vaccine. ...
By combining surface marker profiles with indexes and transcriptomes, the authors now have a map of each cell type that can be used for later identification. They also measured the expression of an additional 352 surface markers that were not used in the original isolation protocol and that might better differentiate cells in the future. Indexed sorting data allowed each ...
Protein-free medium Protein-free media do not contain any protein, only non-protein components. Using protein-free media improves cell growth and protein expression compared to serum-supplemented media, and facilitates downstream purification of any expressed ...
One promising strategy is to deliver genes that express tumor suppressor proteins or anticancer agents into cancer cells to induce cell death or inhibit their proliferation. ...
Differentially expressed proteins refer to proteins that show significant changes in their expression levels in different biological samples or under different treatment conditions. ...
The immune system plays a crucial role in protecting the body from harmful pathogens and foreign invaders. Immune receptors, which are protein molecules expressed on the membrane of immune cells, play a key role in regulating immune responses. ...
Protein-Protein Interaction Sequencing (PPI-Seq) is an emerging technology. Based on high-throughput sequencing, it allows researchers to explore protein interactions at the whole genome level.Technical PrinciplesThe basic principle of Protein-Protein Interaction Sequencing is to use the yeast two-hybrid system ...
Mass Cytometry (CyTOF): This technique allows for the simultaneous measurement of dozens of protein markers in individual cells. By furnishing a detailed protein expression profile, CyTOF helps to understand how different cell populations within the tumor interact and respond to therapies. ...
Collagen, as one of the most abundant proteins in the human body, plays a key role in maintaining the integrity and function of tissue structures. ...
Gene knockout is a procedure that inactivates the function of a specific gene, making it unable to express normally, through genetic engineering techniques. This operation can help us understand the function and role of this gene in an organism. ...
Protein-Protein Interaction databases primarily record experimentally validated protein interaction data. Here are some key Protein-Protein Interaction databases:BioGRID(Biological General Repository for Interaction Datasets)BioGRID is a database focused on biological molecular interactions, including ...
Sequence AlignmentAlign with known protein sequences to identify homologous proteins, which helps to infer protein function and evolutionary relationships.2. ...
Recombinant collagen is a type of collagen protein expressed in microorganisms, animal cells, or plants through genetic engineering technology. ...