Expression System For Protein Expression Articles & Analysis
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In the intricate world of cellular biology, proteins are the undisputed workhorses. They are assembled based on a genetic blueprint, but their story doesn't end there. After synthesis, proteins undergo a vast array of chemical modifications known as post-translational modifications (PTMs). These changes act as molecular switches, fine-tuning a protein's function, localization, and stability. ...
This approach allows for the insertion of genes encoding viral proteins into suitable expression systems such as bacteria, yeast, or mammalian cells. Once synthesized, these proteins spontaneously assemble into VLPs, providing a scalable and efficient way to produce vaccines. ...
Through its impact on translation efficiency and mitochondrial function tRNA methylation modification also promotes tumor cell proliferation by controlling cell cycle-related protein expression. Cell cycle progression within cells is a critical biological function whose stoppage and progression rely entirely on cell cycle-related protein ...
Recombinant protein expression is a cornerstone of modern biopharmaceutical development, providing the means to produce proteins with therapeutic potential, enzymes, and antibodies. ...
On the other hand, IHC is focused on identifying specific proteins within a tissue sample using antibodies. By employing various tags or enzyme-linked systems, IHC enables the visualization of protein expression patterns. ...
Immunohistochemistry: Frozen sections enable the study of protein expression within lung tissues. Specific antibodies can be used to target and visualize proteins associated with inflammatory responses, fibrosis, and cellular proliferation. Molecular Studies: Techniques such as in situ hybridization can be performed on frozen sections to ...
In the rapidly evolving field of cellular biology and genetic engineering, the development of efficient and reliable cell lines is vital for research and therapeutic applications. One such advancement is the establishment of the Monkey DLL3 stable cell line using HEK293T cells. This article provides an overview of the significance of this cell line, its applications, and the methodology behind ...
The principle is to clone one or several antigen genes of a pathogen into host cells, causing them to express the corresponding antigen protein. These proteins are then extracted for use in the vaccine. ...
Cell culture is one of the major technologies in the life sciences. It is a general term used to remove cells, tissues or organs from animals or plants and place them in an artificial environment conducive to their survival and/or proliferation. The basic environmental requirements for optimal cell growth are: controlled temperature, substrate for cell attachment, appropriate growth medium and ...
Human brain has approximately 171 billion cells, of which slightly more than half (approximately 86 billion) are nerve cells. These 86 billion nerve cells are a diverse cell population with hundreds of specialized types and functions, but all originate from three neural cell lineages--neurons, oligodendrocytes and astrocytes. All three cell lineages originate from a pool of neural stem and ...
These approaches allow the targeted delivery of genes that can inhibit tumor growth or enhance the immune system's capability to recognize and destroy cancer cells. One promising strategy is to deliver genes that express tumor suppressor proteins or anticancer agents into cancer cells to induce cell death or inhibit their proliferation. ...
Differentially expressed proteins refer to proteins that show significant changes in their expression levels in different biological samples or under different treatment conditions. Observing such differential expression is crucial for understanding cellular physiology and disease mechanisms. For example, some proteins may be upregulated or downregulated in health and disease states, or between ...
The immune system plays a crucial role in protecting the body from harmful pathogens and foreign invaders. Immune receptors, which are protein molecules expressed on the membrane of immune cells, play a key role in regulating immune responses. ...
Protein-Protein Interaction Sequencing (PPI-Seq) is an emerging technology. Based on high-throughput sequencing, it allows researchers to explore protein interactions at the whole genome level.Technical PrinciplesThe basic principle of Protein-Protein Interaction Sequencing is to use the yeast two-hybrid ...
Brain tumors can be broadly categorized into primary and secondary (or metastatic) tumors. Primary tumors originate in the brain1, while secondary tumors spread from other body parts. Treatment options traditionally include surgery, radiation therapy, and chemotherapy. However, these approaches can be invasive and have severe side effects. The battle against brain tumors is being ...
Collagen, as one of the most abundant proteins in the human body, plays a key role in maintaining the integrity and function of tissue structures. With the development of bioengineering technology, recombinant human collagen (rhCollagen) produced by recombinant DNA technology has been widely applied in various fields such as medicine, cosmetics, and tissue engineering. Therefore, qualitative ...
Gene knockout is a procedure that inactivates the function of a specific gene, making it unable to express normally, through genetic engineering techniques. This operation can help us understand the function and role of this gene in an organism. Gene knockout can be achieved using various methods, including the CRISPR-Cas9 system, TALENs, or ZFNs, etc.Importance ...
Protein-Protein Interaction databases primarily record experimentally validated protein interaction data. Here are some key Protein-Protein Interaction databases:BioGRID(Biological General Repository for Interaction Datasets)BioGRID is a database focused on biological molecular interactions, including protein-protein, protein-DNA, and other types of interaction data.DIP(Database of Interacting ...
Recombinant collagen is a type of collagen protein expressed in microorganisms, animal cells, or plants through genetic engineering technology. ...
After obtaining the protein sequencing results, various analyses can be performed. These analyses help to reveal the function, structure, interactions and roles of the protein in the organism. The commonly used types of analysis mainly include:1. Sequence AlignmentAlign with known protein sequences to identify homologous proteins, which helps to infer protein function and evolutionary ...