genomic sequencing Articles
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Microbial Discovery - Case Study
Goal Understanding of Coronavirus (SARS-CoV-2) of interest using whole-genome sequence data from NCBI Solution Loci helps the scientists to understand the Coronavirus at the molecular level with a few click ...
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Rhabdovirus-like endogenous viral elements in the genome of Spodoptera frugiperda insect cells are actively transcribed: Implications for adventitious virus detection
Highlights We searched the Sf cell genome and transcriptome for adventitious virus sequences. We found rhabdovirus-like sequences transcribed from the Sf cell genome. The rhabdovirus-like sequences are closely related to Sf-rhabdovirus. Transcribed endogenous viral elements may result in false positive adventitious virus detection. Sequencing and comparing both the genome and transcriptome ...
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Whole Genome Sequencing for Rare Diseases
The diagnosis and treatment of rare diseases have long been a daunting challenge due to their complex and often undefined nature. However, with recent advancements in technology, specifically in the realm of genomics, whole genome sequencing (WGS) has emerged as a powerful tool that has revolutionized the diagnosis and management of rare diseases. This groundbreaking technique offers hope to ...
By Protheragen
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Bioinformatics Workflow for Whole Genome Sequencing
Whole genome sequencing (WGS) has the capacity to greatly enhance genomic knowledge and understand mysteries of life by utilizing the most advanced genetic sequencing technologies. WGS can be used for variant calling, genome annotation, phylogenetic analysis, reference genome construction, and more. WGS tries to cover the whole genome, but actually covers 95% of the genome with technical ...
By CD Genomics
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Molecular phylogenetics of Candida Albicans
Candida albicans is the most frequently encountered among the Candida species ssociated with humans as human commensals and opportunistic pathogens (10, 46). Over many years, this pleomorphic yeast has attracted much research attention; it was the first human fungal pathogen for which the full genome sequence was determined (30). C. lbicans is a diploid species without sexual morphology ...
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The Methods of Whole Genome Sequencing
Overview of Whole Genome Sequencing The genome of each individual organism contains its entire genetic information. Whole genome sequencing technology can comprehensively and accurately analyze entire genomes, thereby breaking the information contained in it and revealing the complexity and diversity of the genome. The emergence of whole genome sequencing technology is a revolutionary advancement ...
By CD Genomics
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Applications of Microbial Whole Genome Sequencing
Microorganisms widely exist in nature and are closely related to human life and production. They are generally divided into fungi, actinomycetes, bacteria, spirulina, rickettsia, chlamydia, mycoplasma and viruses. Microbial whole genome sequencing is an important tool for mapping genomes of novel organisms, finishing genomes of known organisms, or comparing genomes across multiple samples. ...
By CD Genomics
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Advances in Targeted Region Enrichment Technologies
What is targeted region sequencing? Whole genome sequencing looks at the whole genome of an organism, while targeted region sequencing looks at only the parts that are of interest. Targeted genome sequencing is a very sensitive and specific way to find SNPs, indels, CNVs, and large SVs. Compared to whole genome sequencing, targeted genome sequencing is more focused, economical, and effective. ...
By CD Genomics
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Genome sequence and characteristics of lrm1, a prophage from industrial lactobacillus rhamnosus strain m1
Prophage Lrm1 was induced with mitomycin C from an industrial Lactobacillus rhamnosus starter culture, M1. Electron microscopy of the lysate revealed relatively few intact bacteriophage particles among empty heads and disassociated tails. The defective Siphoviridae phage had an isometric head of approximately 55 nm and noncontractile tail of about 275 nm with a small baseplate. In repeated ...
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Mitosis, not just open or closed
During cell division, eukaryotic cells must faithfully pass on their genetic material to the next generation during mitosis. It has long been known that lower eukaryotes and higher eukaryotes achieve this in strikingly different ways. Higher eukaryotes undergo an open mitosis in which the nuclear envelope is completely disassembled at the G2/M transition and is not reassembled until after DNA ...
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Viable SARS-CoV-2 Delta variant detected in aerosols in a residential setting with a self-isolating college student with COVID-19
Highlights Viable Delta Variant SARS-CoV-2 collected from air samples in a residential environment. SARS-CoV-2 detected in an area of a single-family dwelling distant from an isolation suite occupied by COVID-19 patient. Assessment of air samples and surface swab samples by rRT-qPCR and cell culture for SARS-CoV-2. Employment of air samplers functioning based on different collection ...
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Sequencing-Based Methods and Techniques for Microbial Identification
Introduction to Microbial Identification Microbiology research revolves around the detection and identification of microorganisms. It can be used in a variety of fields, including the environment, industry, and medicine. Microbial detection and identification techniques have evolved over time, from conventional methods that are based heavily on culture, morphology, physiology, pathology, and ...
By CD Genomics
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Application of Whole Plasmid Sequencing in Analyzing Drug Resistance Genes of Klebsiella pneumoniae
Klebsiella pneumoniae is a significant causative agent of hospital- and community-acquired infections, presenting with various clinical manifestations. Notably, the rapid spread of carbapenem-resistant K. pneumoniae (CRKP) has become a global threat, leading to high morbidity and mortality rates. In this context, researchers have employed high-throughput sequencing to investigate drug resistance ...
By CD Genomics
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Faecalibacillus intestinalis gen. nov., sp. nov. and Faecalibacillus faecis sp. nov., isolated from human faeces.
Abstract Two long-rod-shaped, Gram-stain-positive, obligately anaerobic and non-spore-forming strains, SNUG30099T and SNUG30370T, were isolated from faecal samples of healthy Korean subjects. The strains formed circular ivory-coloured colonies on Brain-heart infusion medium supplemented with 0.5% Difco yeast extract (YBHI) agar and cells were approximately 3.5-4.5×0.3-0.4 µm ...
By KoBioLabs
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Analysis of the genome sequence of lactobacillus gasseri atcc 33323 reveals the molecular basis of an autochthonous intestinal organism
This study presents the complete genome sequence of Lactobacillus gasseri ATCC 33323, a neotype strain of human origin and a native species found commonly in the gastrointestinal tracts of neonates and adults. The plasmid-free genome was 1,894,360 bp in size and predicted to encode 1,810 genes. The GC content was 35.3%, similar to the GC content of its closest relatives, L. johnsonii NCC 533 ...
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What are Vero cells?
Vero cellsare a continuous adherent cell line that is frequently used in cell culture and plays an important role in worldwide vaccine and vector production (manufacturing). Unlike other mammalian cells, they do not secrete interferon alpha or beta when infected by viruses – this is one of the reasons that efficient replication of many viruses can be achieved in Vero cells, ...
By Nuvonis
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Morphology, genome sequence, and structural proteome of type phage p335 from lactococcus lactis
Lactococcus lactis phage P335 is a virulent type phage for the species that bears its name and belongs to the Siphoviridae family. Morphologically, P335 resembled the L. lactis phages TP901-1 and Tuc2009, except for a shorter tail and a different collar/whisker structure. Its 33,613-bp double-stranded DNA genome had 50 open reading frames. Putative functions were assigned to 29 of them. Unlike ...
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Engineering of a human vaginal lactobacillus strain for surface expression of two-domain cd4 molecules
Women are at significant risk of heterosexually transmitted human immunodeficiency virus (HIV) infection, with the mucosal epithelium of the cervix and vagina serving as a major portal of entry. The cervicovaginal mucosa naturally harbors dynamic microflora composed predominantly of lactobacilli, which may be genetically modified to serve as a more efficient protective barrier against the ...
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What Is Single-Cell Sequencing and How to Achieve It?
The cell is the basic unit of life. Most current studies on the human genome, cancer or other fields are still conducted from the population level, and the results are often the mean value of gene expression in cell populations or only represent information on the vital activities of numerically dominant cells, while failing to accurately reflect much of the information on cellular heterogeneity ...
By CD Genomics
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Introduction to Aerobic and Anaerobic Culture
Microorganisms have different growth requirements. When culturing them in the laboratory, it is essential to take note of these conditions because these are needed to be replicated in order to successfully grow microbial cultures in the laboratory. As mentioned in the previous article, one of the conditions needed to be taken into consideration is the oxygen requirement of the microorganisms. ...
By CD Genomics
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