Genomics Approach Long Amplicon Analysis Articles & Analysis
24 articles found
The increasing application of Lactobacillus species in biotechnology, pharmaceutical sciences, and clinical research has necessitated a more nuanced understanding of strain-specific characteristics and formulation parameters. This is particularly relevant for researchers working with lactobacillus acidophilus powder and selecting appropriate lactobacillus strains for specialized applications. ...
Fosmid libraries represent a pivotal advancement in the field of genomics and molecular biology, offering a robust method for genomic cloning and analysis. These libraries are particularly valuable due to their ability to retain large fragments of DNA, making them essential for various applications, including sequencing and functional studies. Understanding Fosmids Fosmids are vector systems ...
Degradome sequencing, also known as parallel analysis of RNA ends (PARE) sequencing, is a cutting-edge technique that leverages high-throughput sequencing and bioinformatics to investigate the degradation products of mRNA. This method is particularly valuable for identifying the target genes regulated by microRNAs (miRNAs), which are small, endogenous non-coding RNAs approximately 22 nucleotides ...
Next-Generation Sequencing (NGS) has revolutionized genomics, providing unprecedented insights into the structure and function of genomes, transcriptomes, and epigenomes. A critical step in the NGS workflow is library preparation, which involves converting DNA or RNA samples into a form that can be sequenced. NGS Library Preparation Kits play a pivotal role in this process, ensuring high-quality ...
Protein Target Quantification aims to accurately measure the abundance of specific proteins in biological samples. It has the advantages of high throughput, accuracy, and reproducibility. Using early data from differential proteomics/transcriptomics/genomics analysis, this method has validated biomarkers in large biological sample sizes. Targeted quantification selectively collects and analyses ...
Introduction to Variant Analysis Rare DNA changes within a population of cells are the first manifestations of mutations, which are the fuel for evolution. The research of genomic variation between species and individual organisms has been revolutionized by next-generation sequencing (NGS) technologies. Variant detection and analysis refer to the sequencing and difference analysis of a genome ...
What is pan-genome? A pan-genome is the sum of all genomic information within a species. With the development of genomic technology, researchers have found that a single reference genome can no longer meet the needs of genomic data analysis, and more and more species, including the human genome, are choosing to construct a pan-genome instead of a single reference genome. Pan-genomes reflect ...
Why need viral vector characterization? Cell and gene therapies for various rare diseases are currently undergoing clinical trials worldwide. The rapid development in this field has led to an increase in regulatory scrutiny and product characterization requirements, as well as a bottleneck in viral vector supply. The manufacturing processes and analytical tools for gene therapy viral vectors need ...
Klebsiella pneumoniae is a significant causative agent of hospital- and community-acquired infections, presenting with various clinical manifestations. Notably, the rapid spread of carbapenem-resistant K. pneumoniae (CRKP) has become a global threat, leading to high morbidity and mortality rates. In this context, researchers have employed high-throughput sequencing to investigate drug resistance ...
Small RNAs are important functional molecules in organisms, which have three main categories: microRNA (miRNA), small interfering RNA (siRNA), and piwi-interacting RNA (piRNA). They are less than 200 nt in length and are often not translated into proteins. Small RNA generally accomplishes RNA interference (RNAi) by forming the core of an RNA-protein complex (RNA-induced silencing complex, RISC). ...
CD Genomics is a world-leading genomics services company that continues to innovate in high throughput sequencing services for the genomics industry, as well as genotyping, bioinformatics, microarrays, and more. The company has recently launched TCR-seq, a powerful service that enables rapid and efficient analysis of T-cell receptor (TCR) diversity and clonality, providing critical support for ...
Whole genome sequencing (WGS) has the capacity to greatly enhance genomic knowledge and understand mysteries of life by utilizing the most advanced genetic sequencing technologies. WGS can be used for variant calling, genome annotation, phylogenetic analysis, reference genome construction, and more. WGS tries to cover the whole genome, but actually covers 95% of the genome with technical ...
Microorganisms widely exist in nature and are closely related to human life and production. They are generally divided into fungi, actinomycetes, bacteria, spirulina, rickettsia, chlamydia, mycoplasma and viruses. Microbial whole genome sequencing is an important tool for mapping genomes of novel organisms, finishing genomes of known organisms, or comparing genomes across multiple samples. ...
Bioinformatics is a discipline developed on the basis of biology, mathematics and computer science. It effectively acquires and analyzes biological data such as nucleic acid sequences and protein structures, so as to conduct comprehensive and accurate biological analysis. Bioinformatics is an extremely important tool to study the microorganisms, which are widespread and highly varied. ...
We are excited to announce a beta release of our Calypso tool on the latest Phase II Undiagnosed Diseases Network (UDN) data. This is a project performed in collaboration with the UDN, and we encourage our initial set of UDN investigators to trial this release and provide any feedback, comments, questions or requests to info@frameshit.io. ...
Introduction Since the completion of the Human Genome Project, genomics has played an increasingly critical role in the biomedical field. Its applications include variation and risk assessment in disease genomics, analysis of changes in transcriptomic gene expression, revealing epigenetic effects on tissue and organ development, and heterogeneity at the single-cell level, among others. We present ...
Introduction to HLA Human leukocyte antigen (HLA) is a 3.6 Mb segment on the short arm of chromosome 6 that contains over 200 genes. It is also known as the major histocompatibility complex (MHC), and it is the most polymorphic region in the human genome, involving diverse immune reactions. HLA is divided into HLA-class I (corresponding to MHC class I) and HLA-class II (corresponding to MHC class ...
Genome initiatives spearheaded by burgeoning numbers of data custodians such as Genomics England (GEL), a Lifebit customer, leverage technological advances to amass large volumes of patient data. As a result, data custodians are veritable gold mines of genome data that could transform precision medicine and provide an equitable solution for diverse healthcare requirements. Pharmaceutical ...
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Introduction to Bioinformatics for Microbial Metagenomics In microbial ecology, metagenomic techniques are now widely utilized to research microbial communities in greater depth, including many strains that cannot be produced in the lab. Microbial bioinformatics may now be used to mine large metagenomic datasets for broad patterns that govern microbial communities. Typical metagenomic and ...
Transfer of lipopolysaccharide from gram-negative bacteria into the systemic circulation of the body leads to the development of endotoxemia, which is detected in cardiometabolic diseases such as cardiovascular disease and obesity in addition to acute infections. Lipopolysaccharide is a virulence factor produced by bacteria, which often triggers systemic inflammation through the body's ...