Genomics S Articles & Analysis
309 articles found
In the realm of genetic engineering and genome editing, single guide RNA (sgRNA) plays a pivotal role, particularly in the context of CRISPR-Cas9 technology. ...
This isn't a simple, one-step affair. It’s a sophisticated, three-tiered enzymatic cascade: Activation (E1): An E1 activating enzyme uses energy (ATP) to "prime" a ubiquitin molecule, making it ready for transfer. ...
Induced pluripotent stem cells (iPSCs) have revolutionized the field of regenerative medicine and cellular biology. They are derived from somatic cells that have been genetically reprogrammed back into an embryonic-like pluripotent state. This transformation enables them to differentiate into any cell type, offering significant potential for disease modeling, drug discovery, and therapeutic ...
Post-transcriptional modifications of RNA molecules include methylation, acetylation deamination, isomerization, glycosylation, thiolation and pseudouracil modification among other types. Methylation represents the predominant post-transcriptional modification found within transfer RNA (tRNA) molecules. tRNA methylation has maintained its evolutionary conservation while also exhibiting dynamic ...
The Adenovirus Production Process Producing adenoviruses involves a series of carefully controlled steps to ensure the final product’s safety and effectiveness. The production process typically includes: Cell Line Development: A suitable cell line, often HEK293 cells, is chosen for the replication of the adenovirus. ...
In the realm of drug discovery, kinase screening services have emerged as a pivotal tool for understanding complex cellular signaling pathways and identifying potential therapeutic targets. Kinases are enzymes that play a crucial role in regulating cellular functions by adding phosphate groups to proteins, a process known as phosphorylation. This post-translational modification can activate or ...
In recent years, the field of gene editing has experienced groundbreaking advancements, with CRISPR/Cas9 emerging as a powerful technology for precise genetic manipulation. Among various delivery systems, lentiviruses have garnered significant attention due to their ability to introduce genetic material into both dividing and non-dividing cells, making them an ideal vehicle for gene therapy ...
The Type II restriction endonuclease HindII (from Haemophilus influenzae Rd) remains a versatile molecular tool despite being one of the earliest restriction enzymes characterized. While many researchers now default to enzymes with longer recognition sequences for routine cloning, HindII enzyme activity characteristics provide distinct advantages in specialized applications including methylation ...
While commercial literature often simplifies these distinctions, research-grade applications demand precision in both strain selection and formulation properties. Genomic Heterogeneity Within L. acidophilus: Implications for Research The taxonomic designation Lactobacillus acidophilus encompasses significant genomic diversity, with strain-level variations that ...
The Type II restriction endonuclease HindII (from Haemophilus influenzae Rd) remains a versatile molecular tool despite being one of the earliest restriction enzymes characterized. While many researchers now default to enzymes with longer recognition sequences for routine cloning, HindII enzyme activity characteristics provide distinct advantages in specialized applications including methylation ...
These subgroups interact in a complex signaling network to decide a cell’s fate. Clinical Importance and Research Highlights One of the most significant areas of BCL2-related cancer research involves the development of BCL2 inhibitors. ...
These cells are of significant interest in oncology due to their potential to provide vital information about the tumor’s characteristics and behavior. One of the advanced techniques used to analyze CTCs is Fluorescence In Situ Hybridization (FISH). ...
Advancements in medical instruments are revolutionizing healthcare, enhancing precision in diagnostics and surgical procedures. The quantification of genomic DNA is shaping personalized medicine, while tissue oxygenation imaging improves patient monitoring. Innovations like the disposable linear stapler and aspirator endocervical optimize surgical efficiency, particularly in procedures involving ...
In the ever-evolving landscape of cancer research, two intriguing concepts have emerged that are reshaping how scientists approach treatment: synthetic lethality and parthanatos. Both concepts encapsulate the complexity of cellular processes and open new avenues for targeted therapies. What is Synthetic Lethality? Synthetic lethality occurs when the simultaneous disruption of two genes ...
Preclinical drug discovery represents a critical phase in the development of new therapeutics. It is the process that bridges the gap between basic research and clinical testing, allowing for the evaluation of potential drug candidates in biological systems before they are tested in humans. Central to this phase is the use of appropriate disease models, which are essential for understanding the ...
Methodologies for Creating Custom Knockout Cell Lines CRISPR-Cas9 Technology: CRISPR-Cas9 has revolutionized genome editing due to its simplicity and efficiency. Researchers design guide RNA (gRNA) that directs the Cas9 nuclease to a specific genomic location. Once the target gene is located, Cas9 induces a double-strand break, leading to disruptions in the ...
Traditional therapies often lack the specificity needed to target cancer cells while preserving healthy tissues accurately. Alfa Cytology’s cancer immunotherapy development services are designed to tackle these obstacles by offering tailor-made, high-quality immunotherapeutic solutions that align with the unique immunological profile of different cancers. ...
Double-stranded RNA (dsRNA) has gained significant attention in the fields of molecular biology, genetics, and therapeutics due to its pivotal role in gene silencing and the modulation of cellular processes. The production of dsRNA can be achieved through various methods, each offering unique advantages depending on the desired application. This article delves into the primary techniques for ...
Fosmid libraries represent a pivotal advancement in the field of genomics and molecular biology, offering a robust method for genomic cloning and analysis. These libraries are particularly valuable due to their ability to retain large fragments of DNA, making them essential for various applications, including sequencing and functional studies. Understanding Fosmids Fosmids are vector systems ...
Genome editing, a transformative technology in genetic research, has revolutionized the way scientists study and modify plant genomes. Among the most prominent methods utilized in recent years, CRISPR-Cas9 has emerged as a breakthrough tool that allows for precise and targeted modifications. Model plants, often employed due to their well-characterized genomes and simple genetics, serve as ideal ...