Hplc Articles & Analysis: Older
33 articles found
High-Performance Liquid Chromatography (HPLC)(1) HPLC can be used to quantitatively analyze various components in the medium, such as amino acids, sugars, vitamins, and other small molecules.(2) This technique provides high resolution and can separate and quantify individual components in complex mixtures.2. ...
For instance, High-Performance Liquid Chromatography (HPLC) and Capillary Electrophoresis (CE) can be used to analyze the types and quantities of proteins and amino acids; Gas Chromatography (GC) and Mass Spectrometry (MS) can be used to analyze the composition of sugars and lipids; various biochemical analysis methods can be used to measure the content of growth factors and ...
ChromatographyHigh-performance liquid chromatography (HPLC) can be used to separate and quantify different types of collagen.2. ...
Here are some commonly used methods for determining collagen content.ChromatographyHigh-performance liquid chromatography (HPLC) is a commonly used method for quantitatively analyzing collagen and other proteins. ...
High Performance Liquid Chromatography (HPLC)HPLC is a commonly used technology that can be used to separate and quantify glycation products in collagen.3. ...
Among them, High-Performance Liquid Chromatography (HPLC) is the most commonly used detection method, which can effectively separate and quantitatively analyze glycosylated impurities, with simple operation and good repeatability. ...
High-Performance Liquid Chromatography (HPLC) HPLC is a versatile analytical technique used to separate, identify, and quantify components in a mixture. For ADCs, HPLC can be used to analyze the distribution of drug molecules on the antibody, assess the purity of the conjugate, and detect free drug. Variants like size-exclusion chromatography ...
Glycan SeparationTechniques such as High-Performance Liquid Chromatography (HPLC) or electrophoresis are used to separate glycans.3. Glycan Structure IdentificationMethods like Mass Spectrometry (MS) or Nuclear Magnetic Resonance (NMR) are used to analyze the structure of the separated glycans.4. ...
Therefore, detailed analysis and control of the monosaccharide content and types in recombinant protein vaccines are very important.To analyze the content and types of monosaccharides in recombinant protein vaccines, technologies such as High-Performance Liquid Chromatography (HPLC) and Mass Spectrometry (MS) are usually required. HPLC can perform accurate ...
Researchers often use chromatography techniques, such as high-performance liquid chromatography (HPLC) or gas chromatography (GC), to separate and analyze the extracted compounds based on their chemical properties. ...
Gel Permeation Chromatography (GPC) or High-Performance Liquid Chromatography (HPLC)Estimate the molecular weight of the sample by comparing the elution time of known molecular weight standards.2. ...
Accurate determination of its concentration is crucial for multiple fields such as biomaterial research, food industry, and pharmaceutical research.Liquid Chromatography (LC) is an efficient and precise analytical technique for collagen concentration measurement, especially High-Performance Liquid Chromatography (HPLC) and Ultra-High Performance Liquid Chromatography (UHPLC) ...
High-Performance Liquid Chromatography (HPLC)HPLC is a method used for the separation and quantitative analysis of proteins and peptide segments. ...
Sirius red binds to the collagen in the solution, and the collagen is quantified by measuring its absorbance.Determination of Hydroxyproline Residue After Sample Hydrolysis (by Colorimetric Kit or HPLC)The residual amount of hydroxyproline is determined by colorimetric kit or high-performance liquid chromatography (HPLC). ...
High Performance Liquid Chromatography (HPLC)(1) Specific amino acid content in the sample can be analyzed using HPLC to calculate the collagen content.(2) Acid hydrolysis is usually required in the sample treatment step.6. ...
Complete Variety: Over 30 target proteins including IL-17A/B/C/D/E/F, IL-17A&17F, and IL-17RA/RB/RC/RD/RE can be selected according to needs; Available Labels: His/Fc/Biotin High Purity: Verified by SDS-PAGE/SEC-HPLC High Activity: Verified by ELISA/BLI/Cell Related Products IL-17A Recombinant Human IL-17A, tag free Recombinant Human IL-17A (C-6His) Recombinant Mouse IL-17A ...
Herein, we provide an overview of the currently popular protein quantification technologies and their application in clinical research. Introduction of HPLC-MS/MS-based Proteomics Technology The development of high-performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) based proteomics technology mainly depends on the liquid chromatography-mass ...
In addition to fume hoods, rotary evaporators, and glassware, we are able to use major equipment including a Bruker Avance 300 MHz NMR spectrometer, Agilent 1100 LCMS, preparative HPLC systems, automatic flash chromatography system, hydrogenator, and ozone ...
Quality control of hair, skin, and dentalcare products continues to increase in importance for producers. Aside from safeguarding our health, it is equally important to avoid the sale of out-of-specification products. Product recalls are very expensive and have a lasting effect on a company’s reputation. Therefore, intensive testing of these different care products is essential before ...
For example, for solid oral preparations, several kinds of excipients can be selected at first, and then mix the main drug and excipients with a certain proportion, and put them under strong light (4500±500Lux), high temperature (60℃) and high humidity (90±5 %) conditions for 10 days, and finally use HPLC to check whether the content and related substances changed ...