Immunoblot Articles & Analysis
6 articles found
Then, through protein electrophoresis, the target protein and its binding proteins are detected by immunoblotting technology.Analysis Workflow1. Cell Dissolution and Protein ExtractionRIPA and other lysis buffers are often used to break the cells and extract as much protein as possible under low-temperature conditions.2. ...
From protein immunoblotting to immunofluorescence, and then to FRET, antibodies are often an important determinant of successful and repeatable experiments. ...
Currently, detecting LC3 by immunoblotting or immunofluorescence has become a reliable method for monitoring autophagy and autophagy-related processes, including autophagic cell death. ...
Protein ElectrophoresisSeparate the precipitated proteins by SDS-PAGE.4. Immunoblot AnalysisPerform immunoblotting (Western Blot) analysis using antibodies against ubiquitin to detect whether Tau protein is ubiquitinated.5. ...
Moreover, experimental methods to validate the predicted results (such as mass spectrometry, immunoprecipitation, and immunoblotting) are also indispensable parts of the research ...
Patients with a suspected Bartonella infection are now able to order the IGeneX ImmunoBlots. ImmunoBlots use recombinant antigens to increase sensitivity, specificity and extend species coverage. They effectively replace the Western blot. ImmunoBlots are already available for other diseases including Lyme and Tick-Borne Relapsing Fever (TBRF). ...
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