In Vitro Assays Articles & Analysis
20 articles found
The recombinant form of ELANE protein is typically expressed in eukaryotic systems to preserve post-translational modifications and enzymatic activity. Activity assays, such as chromogenic peptide cleavage or fluorogenic substrate kinetics, are used to validate protein functionality post-purification. ...
Recognizing the need for a more accurate and efficient evaluation system, the Comprehensive in vitro Proarrhythmia Assay (CiPA) initiative emerged. At the heart of this initiative are CiPA Translational Assays, designed to improve the prediction of drug-induced cardiac arrhythmias. ...
In vitro permeability assay kits play a crucial role in the field of pharmaceutical development, particularly when evaluating the absorption characteristics of drugs. ...
Cells for use in plate cultures or suspension assays represent a key tool for predicting properties such as hepatotoxicity, metabolism, and potential drug-drug interactions. ...
The Clinical CRISPR Successes Are Stacking Up. But Where’s the Emphasis on Quality Control? In mid-September, Intellia released some promising data on two of their CRISPR-based therapeutics. These results are a significant milestone for genome editing and validation of its effectiveness in the clinic. However, the safety of CRISPR-based therapeutics has been a major concern and part of a ...
Paxlovid Also Reduces Hospitalization/Morbidity Death in Non-High-Risk Unvaccinated/High-Risk Vaccinated COVID-19 Patients In an interim analysis of EPIC-SR, Paxlovid reduced hospitalization/mortality in non-high-risk unvaccinated/high-risk vaccinated COVID-19 patients by 70% compared to the placebo group; and no patients in the treatment group died of disease. In Vitro ...
In addition, six drug candidates demonstrated antiviral effects by in vitro drug screening assays. Researchers have discovered a powerful drug candidate, methotrexate, which inhibits viral replication. ...
Overview Confirm Cas9 mRNA expression With our ready-to-use Cas9 RT-PCR primer set, you can quickly and easily confirm Cas9 expression in cells or in vitro transcription assays. The amplicon for Game 1 is 219 bp and the amplicon for Game 2 2 is 122 bp. ...
The initially obtained lead compound 1 showed excellent binding in the in vitro target binding assays, but its affinity plummeted by nearly 70 times in cellular assays, which may be caused by the high concentration of ATP in the cell binding to its competing target. ...
In this study, we isolatedLactobacillus species from healthy Korean women and investigated their antifungal effects againstC.albicans in vitro andin vivo.Lactobacillus conditioned supernatant (LCS) ofL.crispatus andL.fermentum inhibitedC.albicans growthin vitro. ...
We have successfully identified small molecule antagonists against PCSK9 through a virtual screen coupled with in vitro and cell-based assays. Our lead compound, P-4, exhibited a concentration-dependent inhibition of the PCSK9/LDLR interaction with an IC50 in the nanomolar range, an increase in the level of LDLR in recombinant cell-based assays, ...
The US Environmental Protection Agency has responsibility for assessing endocrine activity of more than 10 000 chemicals, a task that cannot reasonably be achieved solely through use of available mammalian and nonmammalian in vivo screening assays. Hence, it has been proposed that chemicals be prioritized for in vivo testing using data from in vitro ...
Coumarin derivative anticoagulant rodenticides used for rodent control are posing a serious risk to wild bird populations. For warfarin (WF) a classic coumarin derivative, chicken have a high LD50 while mammalian species generally have much lower LD50. Large interspecies differences in sensitivity to WF are to be expected. We previously reported substantial differences in WF metabolism among ...
Changes in the endocrine potency of municipal wastewater at 3 wastewater treatment plants (WWTPs) in Australia were investigated using a panel of in vitro receptor‐driven transactivation assays. The assays were based on human estrogen receptor α, androgen receptor, progesterone receptor, glucocorticoid receptor, and peroxisome ...
In vitro biotransformation assays are currently being explored to improve estimates of bioconcentration factors of potentially bioaccumulative organic chemicals in fish. ...
TBECH has been detected in the environment and in previous in silico and in vitro studies we have shown that TBECH can activate both the human androgen receptor (hAR) and the zebrafish AR (zAR) suggesting that it is a potential endocrine disruptor. The present study was aimed at determining the interaction of TBECH with the chicken AR (cAR). TBECH bound to cAR but in ...
We investigated the endocrine‐disrupting effects of PFOS using a combination of in vitro and in vivo assays. Reporter gene assays were used to detect receptor‐mediated (anti‐)estrogenic, (anti‐)androgenic, and (anti‐)thyroid hormone activities. ...
Lymphocyte proliferation was measured in vitro in adult and prefledgling gulls. Reference data came from the Great Lakes and Bay of Fundy. ...
The aim of this study was to evaluate the cytogenetic effect of radio-labelled octreotate in blood cells in vitro, using the cytokinesis-block micronucleus assay. Blood samples of healthy donors were exposed to different activities of octreotate labelled with 131I (n = 3) and 177Lu (n = 3), where radioactive concentration ranged from 600 to 5600 kBq/mL. ...
As shown by the results of the lung-colony assay the number of tumour nodules was significantly reduced in the animals exposed to 0.2 Gy in comparison to those irradiated with 0.5 and 1.0 Gy X-rays. ...