in vitro culture Articles
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Efficacy of antioxidant overproduction on fiber growth and maturation in cotton
Electron transport in plant cells inevitably results in the creation of reactive oxygen species (ROS) that can hinder metabolic processes and cause cellular damage. In spite of the gains additional antioxidants may impart to the cotton (Gossypium hirsutum L.) photosynthetic apparatus, single time point measurements on live plants have not reflected distinguishable phenotypes for key physiological ...
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New trends in the management of late radiation morbidity
Currently, no reliable methods and no suitable marker profile exist that could predict radiosensitivity in patients. This paper describes the irradiation response of lymphocytes, fibroblasts and tumour biopsies cultured in vitro, clonogenic survival at 2 Gy (SF2), chromosomal aberrations, and CD4 and CD8 lymphocyte apoptosis. The results, and other accumulating evidence, strongly suggest that ...
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Novel Cas9 Nuclease Aids in the Safety of Gene Editing
CRISPR/Cas9 gene editing technology has been widely used in many fields since its inception. Gene therapy developed using this technology has huge prospects in the medical and health fields. CRISPR/Cas9 technology enables gene-editing of the genome at specified sites, but a common worry about the technology is that gene-editing occurs where it shouldn't. In recent years, researchers at the ...
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A Closer Look at Metabolic Disease Models and Diabetes Models
Diabetes, specifically Type 1 and Type 2 Diabetes Mellitus, remains one of the most commonly encountered metabolic diseases worldwide. The complexity and diversity of these conditions necessitate the development of detailed medical models for a comprehensive understanding of the diseases. Over time, Type 1 and Type 2 Diabetes Mellitus Models, Metabolic Disease Models, and generalized Diabetes ...
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Augmentation of anti–tumour activity of cisplatin by pectin nano–conjugates in B–16 mouse model: pharmacokinetics and in–vivo biodistribution of radio–labelled, hydrophilic nano–conjugates
Nanoconjugates have matured from simple devices to multifunctional, biodegradable, non–toxic and non–immunogenic constructs, capable of delivering synergistically functioning drugs in vivo. The present study evaluates the efficacy of drug polymer self folding nano–conjugates of pectin–cisplatin to enhance blood circulating levels of cisplatin. Physical characterisation was done by DLS, zeta ...
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How to activate the "first responders" of the immune system - NK cells
About NK Cells Natural Killer (NK) cells play a critical role in the innate immune surveillance against cancer and viral infections. They are the "first responders" capable of spontaneously recognizing abnormal cells in the body, swiftly eliminating them through targeted cytotoxic mechanisms, and effectively producing pro-inflammatory cytokines and chemokines to recruit and activate other immune ...
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Applications of Cell-penetrating Peptides
Cell-penetrating peptides (CPPs) are short peptides that facilitate cellular intake and uptake of molecules ranging from nano-size particles to small chemical compounds to large fragments of DNA. As a versatile peptide, CPP has been widely used for diagnostic and therapeutic applications. 1. Oligo and siRNA Transport As an important means for targeted therapy of specific genes, oligonucleotides ...
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Classes and Applications of Cell-Penetrating Peptides
Classes of Cell-Penetrating Peptides There are many types of cell-penetrating peptides (CPPs), and their classification is based on physicochemical properties, sources, intake mechanisms, and biomedical applications. There is no unified conclusion yet. According to their physical and chemical properties, CPPs can be divided into three types: cationic, amphiphilic and hydrophobic, among which ...
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Recapitulating the Multifaceted Inflammatory Environment of the Intestine In Vitro
Intestinal inflammatory diseases are highly complex, which has thus far prevented development of an effective in vitro disease model. With access to a donor-derived cell biobank and the ability to recapitulate region-specific environments of the intestine, Altis Biosystems is working toward the development of an in vitro RepliGut® model to accurately recapitulate the complex intestinal ...
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The Crucial Role of Cytokines in Biomedical Research and Drug Development
Low Endotoxin Cytokines Matters Organoid culture, CAR-T cell therapy, stem cell therapy, and other topics have become broadly discussed among biomedical researchers. In fact, it is not difficult to see that these advanced technologies are all related to cell culture. In order to maintain efficient growth, proliferation, and differentiation during in vitro cell culture, many cell cultures require ...
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Evaluating Microbiome Therapies with Intestinal Cell Models
The microbiome has been shown to play roles in many different disease processes and is implicated in many more, from infectious and gastrointestinal diseases to autoimmune and neurological disorders. Advanced models of the gut leveraging intestinal and colonic stem cells are enabling the development of more reliable and robust systems for evaluating the impact of changes in the microbiome on gut ...
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Study of the pathogenic mechanisms of autoimmune myasthenia gravis - Case Study
Contexte Myasthenia gravis (MG) is an autoimmune disease caused by the presence of antibodies directed against components of the muscle membrane located at the neuromuscular junction. In the majority of cases, these are autoantibodies directed against the acetylcholine receptor (AChR). The origin of the autoimmune response is not known, but thymic abnormalities and defective regulation of the ...
By Inovarion
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Examining Models of Chronic Obstructive Pulmonary Disease (COPD) and their Application in Other Disease Frameworks
The field of medical science has long utilized disease models, which are systems simulating the progression and expression of a disease, to better understand various illnesses. These models facilitate the study of disease pathology, identifying underlying mechanisms, and developing potential treatments. In the context of respiratory diseases, numerous models have been developed, with a prominent ...
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Examining Models of Chronic Obstructive Pulmonary Disease (COPD) and their Application in Other Disease Frameworks
The field of medical science has long utilized disease models, which are systems simulating the progression and expression of a disease, to better understand various illnesses. These models facilitate the study of disease pathology, identifying underlying mechanisms, and developing potential treatments. In the context of respiratory diseases, numerous models have been developed, with a prominent ...
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Pooled Human Cryopreserved Hepatocytes
Hepatocytes are the chief functional cells of the liver, responsible for the selective uptake, metabolism and excretion of most drugs. Cells for use in plate cultures or suspension assays represent a key tool for predicting properties such as hepatotoxicity, metabolism, and potential drug-drug interactions. Since these cells mimic the properties of the liver, researchers can evaluate the ...
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