in vitro results Articles
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CYP‐mediated warfarin metabolic ability is not a critical determinant of warfarin sensitivity in avian species; in vitro assays in several birds and in vivo assays in chicken
Coumarin derivative anticoagulant rodenticides used for rodent control are posing a serious risk to wild bird populations. For warfarin (WF) a classic coumarin derivative, chicken have a high LD50 while mammalian species generally have much lower LD50. Large interspecies differences in sensitivity to WF are to be expected. We previously reported substantial differences in WF metabolism among ...
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Part 1: In-vitro release of gentamicinpalmitate coating in uncemented titanium implants
Microporous surfaces of commercially available uncemented Tilastan® implants were coated with gentamicinpalmitate. The roughness of the titanium implant surfaces remained largely intact. During the first few days, the gentamicin release was comparable to the elution of this antibiotic from commercially available PMMA bone cements. Compared to PMMA cements, the implants coated with the ...
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Transcriptional Regulation of xyn2 in Hypocrea jecorina
The xylanase system of the filamentous fungus Hypocrea jecorina (Trichoderma reesei) consists of two specific xylanases, Xyn1 and Xyn2, which are simultaneously expressed during growth on xylan but respond differentially to low-molecular-weight inducers. Using in vivo footprinting analysis of xylan-induced and noninduced mycelia, we detected two adjacent nucleotide sequences (5'-AGAA-3' on the ...
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TOWARDS IMPROVED MODELS FOR PREDICTING BIOCONCENTRATION OF WELL‐METABOLIZED COMPOUNDS BY RAINBOW TROUT USING MEASURED RATES OF IN VITRO INTRINSIC CLEARANCE
Models were developed to predict the bioconcentration of well‐metabolized chemicals by rainbow trout. The models employ intrinsic clearance data from in vitro studies with liver S9 fractions or isolated hepatocytes to estimate a liver clearance rate which is extrapolated to a whole‐body biotransformation rate constant (kMET). Estimated kMET values are then used as inputs to a mass‐balance ...
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Analysis of the genome sequence of lactobacillus gasseri atcc 33323 reveals the molecular basis of an autochthonous intestinal organism
This study presents the complete genome sequence of Lactobacillus gasseri ATCC 33323, a neotype strain of human origin and a native species found commonly in the gastrointestinal tracts of neonates and adults. The plasmid-free genome was 1,894,360 bp in size and predicted to encode 1,810 genes. The GC content was 35.3%, similar to the GC content of its closest relatives, L. johnsonii NCC 533 ...
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Inhibition of the development of pulmonary tumour nodules and stimulation of the activity of NK cells and macrophages in mice by single low doses of low-LET radiation
As indicated by a few recent reports, exposures to low doses of ionising radiation can trigger the activity of natural anti-tumour defence mechanisms and impede the development of transplantable tumours in rodents. In our experimental system, BALB/c mice were irradiated with a single dose of 0.1, 0.2, 0.5, or 1.0 Gy X-rays and then i.v. injected with L1 sarcoma cells. As shown by the results of ...
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Efficacy of antimicrobial polymer coatings in an animal model of bacterial infection associated with foreign body implants
Abstract Objectives: To assess support discs, comprising polyethylene terephthalate (PET), coated with different polymer/levofloxacin combinations for antimicrobial activity in an animal model of infection, in order to explore the use of specific polymer coatings incorporating levofloxacin as a means of reducing device-related infections. Methods: Aliphatic polyester-polyurethanes ...
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Developing a Robust in Vitro Intestinal Model for Preclinical Candidate Screening of GI Toxicity
Gastrointestinal (GI) issues are common side effects of many drugs. Anticipating such side effects before clinical trials is limited by a lack of effective models for evaluating preclinical candidates for their potential to cause diarrhea, nausea, and other gut-related side effects. With its RepliGut® in vitro human intestinal model, Altis Biosystems is developing a system that could help ...
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Research Progress of Tetrahydrocurcumin
Tetrahydrocurcumin (THC), as the most active and main metabolite produced in the metabolism of curcumin in vivo, can be isolated from the cytoplasm of the small intestine and liver after curcumin administration in human or mouse. Tetrahydrocurcumin can effectively inhibit tyrosinase, inhibit the generation of Oxygen Free Radicals and scavenge the formed free radicals. It has obvious antioxidant ...
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Endocrine disrupting effect of the UV filter benzophenone‐3 in zebrafish, Danio rerio
The chemical UV filter benzophenone‐3 (BP‐3) is suspected to be an endocrine disruptor based on results from in vitro and in vivo testing. However, studies including endpoints of endocrine adversity are lacking. The present study investigated the potential endocrine disrupting effects of BP‐3 in zebrafish (Danio rerio) in the Fish Sexual Development Test (OECD TG 234) and a 12 day adult male ...
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Methods for Protein-Protein Interaction Research
Proteins are the most direct performers of biological functions. Although some proteins can accomplish their mission independently, most of them require the assistance of some chaperone molecules to complete the task together or form a complex before they can fully perform their functions. Therefore, understanding the interaction between protein and protein can help us better understand the ...
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New structural target against COVID-19
A new structural target for drug development against COVID-19 has been unveiled today by Sibylla Biotech in collaboration with the Italian National Institute of Nuclear Physics (INFN), the University of Trento and University of Perugia.The breakthrough drug discovery strategy pioneered by Sibylla in this study would not only promise to reduce the risk of drug resistance and harmful side effects, ...
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Why Liver Health is so important? (Or, did you know that in the U.S. 100 million people may have fatty livers?)
Let's face it; the liver is the primary organ in the body that often gets overlooked when it comes to health. According to Traditional Chinese Medicine, the liver is the organ responsible for the smooth flow of emotions, as well as Qi (Energy) and blood. It is the organ that is most affected by excess stress or emotions.Ii] The liver is responsible for numerous functions in the body. Its primary ...
By Algatech
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Development of a Bioartificial Vascular Pancreas
Abstract Transplantation of pancreatic islets has been shown to be effective, in some patients, for the long-term treatment of type 1 diabetes. However, transplantation of islets into either the portal vein or the subcutaneous space can be limited by insufficient oxygen transfer, leading to islet loss. Furthermore, oxygen diffusion limitations can be magnified when islet numbers are increased ...
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Based on Antibody Targeted Protein Degradation
In recent years, PROteolysis TArgeting Chimeras (PROTAC), which utilizes the cell's own degradation mechanisms to eliminate specific disease-related proteins, has emerged as one of the most promising methods. Apart from PROTAC, various targeted protein degradation (TPD) strategies are emerging, involving molecular glue, Autophagy-Targeting Chimera (AUTEC), Autophagosome Tethering Compound ...
By BOC Sciences
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Vaginal lactobacilli inhibit growth and hyphae formation of Candida albicans
Abstract Lactobacillusspecies are the predominant vaginal microbiota found in healthy women of reproductive age and help to prevent pathogen infection by producing lactic acid, H2O2 and anti-microbial compounds. Identification of novel vaginalLactobacillus isolates that exhibit efficient colonisation and secrete anti-Candida factors is a promising strategy to prevent vulvovaginal candidiasis. ...
By KoBioLabs
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