in vitro use Articles
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Mbd4-mediated glycosylase activity on a chromatin template is enhanced by acetylation
The ability of the MBD4 glycosylase to excise a mismatched base from DNA has been assessed in vitro using DNA substrates with different extents of cytosine methylation, in the presence or absence of reconstituted nucleosomes. Despite the enhanced ability of MBD4 to bind to methylated cytosines, the efficiency of its glycosylase activity on T/G mismatches was slightly dependent on the extent of ...
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Microencapsulation of bacteriophage felix o1 into chitosan-alginate microspheres for oral delivery
This paper reports the development of microencapsulated bacteriophage Felix O1 for oral delivery using a chitosan-alginate-CaCl2 system. In vitro studies were used to determine the effects of simulated gastric fluid (SGF) and bile salts on the viability of free and encapsulated phage. Free phage Felix O1 was found to be extremely sensitive to acidic environments and was not detectable after a ...
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Liposome encapsulated gold nanoshells for NanoPhototherapy induced hyperthermia
Gold nanoshells have been extensively studied since their invention and their role in both in vitro and in vivo photothermal therapy has been explored and demonstrated. In this work, we investigate liposomal delivery of gold nanoshells and evaluate their effects for in vitro NanoPhototherapy induced hyperthermia in human mammary carcinoma cells. In addition, we compare the application of liposome ...
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TBECH‐mediated steroid hormone receptor activation and gene regulation in chicken LMH cells
The incorporation of brominated flame retardants into industrial and household appliances has increased their occurrence in the environment resulting in deleterious effects on wildlife. With the increasing restraints on available compounds there has been a shift to using BFRs that has seen the production of alternative BFRs such as 1,2‐dibromo‐4‐(1,2 dibromoethyl) cyclohexane (TBECH). TBECH ...
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Cell-Penetrating Peptides: Features, Mechanisms, Applications
Cell-penetrating peptides (CPPs) refer to water-soluble, partly hydrophobic, and/or polybasic short peptides that are fewer than 30 amino acids in size. Different types of CPPs like chimeric peptides and hydrophobic peptides have different lengths, charges, solubility, and hydrophobicity. At current, cell-penetrating peptides are often classified in line with their characteristics, including ...
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An in vitro method for determining the bioaccessibility of pharmaceuticals in wildlife
Wildlife can be exposed to human pharmaceuticals via prey that have accumulated the compounds from wastewater, surface water, sediment and soil. One factor affecting internal absorption of pharmaceuticals is bioaccessibility, the proportion of the compound that enters solution in the gastrointestinal tract. Currently, the bioaccessibility of most pharmaceuticals in prey remains unknown for ...
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Novel Cas9 Nuclease Aids in the Safety of Gene Editing
CRISPR/Cas9 gene editing technology has been widely used in many fields since its inception. Gene therapy developed using this technology has huge prospects in the medical and health fields. CRISPR/Cas9 technology enables gene-editing of the genome at specified sites, but a common worry about the technology is that gene-editing occurs where it shouldn't. In recent years, researchers at the ...
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Fabrication And Characterization Of 3D Bioprinted Triple-Layered Human Alveolar Lung Models
Researchers from Nanyang Technological University and its Center for 3D printing (SC3DP) have developed a 3D printed a highly repeatable and scalable three-dimensional in-vitro alveolar lung model using our REGENHU bioprinter, that could be exploited for pathogen translocation studies and respiratory-related toxicological testing applications. The triple-cell layered lung model was realized ...
By Regenhu
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CD Biosciences Launches Substrate-Chromogens for Immunohistochemistry
CD BioSciences, a US-based biotechnology company focusing on the development of imaging technology, has announced the launch of its innovative line of Chromogenic Substrates for immunohistochemistry (IHC). These cutting-edge substrates offer superior signal sensitivity, long-term storage stability, enhanced safety, and user-friendliness, empowering researchers to unlock deeper insights into ...
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The Power Of RepliGut in Vitro Intestinal Stem Cell Models
Both current in vitro and in vivo models used in the development of candidate therapies for the treatment of intestinal diseases have severe limitations. RepliGut®, an in vitro human intestinal epithelial stem cell model, addresses many of these issues and has significant potential to reduce the time and cost of drug development. This model may even help to identify optimal therapies for ...
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Examining Models of Chronic Obstructive Pulmonary Disease (COPD) and their Application in Other Disease Frameworks
The field of medical science has long utilized disease models, which are systems simulating the progression and expression of a disease, to better understand various illnesses. These models facilitate the study of disease pathology, identifying underlying mechanisms, and developing potential treatments. In the context of respiratory diseases, numerous models have been developed, with a prominent ...
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Examining Models of Chronic Obstructive Pulmonary Disease (COPD) and their Application in Other Disease Frameworks
The field of medical science has long utilized disease models, which are systems simulating the progression and expression of a disease, to better understand various illnesses. These models facilitate the study of disease pathology, identifying underlying mechanisms, and developing potential treatments. In the context of respiratory diseases, numerous models have been developed, with a prominent ...
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Increasing the Efficiency of Drug Development with Preclinical Testing Using Human Intestinal Stem Cells
Due to a lack of robust in vivo gut models, animal studies are generally required to evaluate gut toxicity. However, these studies can be lengthy and expensive and may not accurately recapitulate the behavior of the human gastrointestinal tract. Drugs are thus often developed with undesired gut side effects that are not apparent until clinical trials. Organ-on-a-chip models are the closest ...
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The Liposome-Based PROTAC Strategy
Proteolysis Targeting Chimeras (PROTACs) are a novel approach for degrading target proteins. Recently, a new split-and-mix PROTAC (SM-PROTAC) system based on liposome self-assembly, known as LipoSM-PROTAC, was reported in the Journal of the American Chemical Society. The LipoSM-PROTAC system exhibits selective degradation of target proteins, with folate serving as a key ligand. In this study, the ...
By BOC Sciences
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A Closer Look at Reporter Lentiviral Particles: Tool for Biomedical Research
Introduction For years, scientists have leveraged the brilliant potentialities of genetic science to explore how cells function and interact in living organisms. Among the numerous tools employed are "reporter systems," which use a visible marker to track gene expression, protein interactions, and other vital biological processes. One such system employs reporter lentiviral particles, using the ...
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Study of the pathogenic mechanisms of autoimmune myasthenia gravis - Case Study
Contexte Myasthenia gravis (MG) is an autoimmune disease caused by the presence of antibodies directed against components of the muscle membrane located at the neuromuscular junction. In the majority of cases, these are autoantibodies directed against the acetylcholine receptor (AChR). The origin of the autoimmune response is not known, but thymic abnormalities and defective regulation of the ...
By Inovarion
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A hybrid modeling approach for assessing mechanistic models of small molecule partitioning in vivo using a machine learning integrated modeling platform
Prediction of the first-in-human dosing regimens is a critical step in drug development and requires accurate quantitation of drug distribution. Traditional in vivo studies used to characterize clinical candidate’s volume of distribution are error-prone, time and cost intensive and lack reproducibility in clinical settings. The paper demonstrates how a computational platform integrating ...
By VeriSIM Life
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Medical Device Regulation - Indian Perspective
The Indian Medical Devices industry is mostly import-dependent with no specific regulations in place till recent times. With the introduction of MDR (Medical Device Rules 2017) attempt has been made to harmonize the Indian medical device Industry as per globally prevailed ...
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TOWARDS IMPROVED MODELS FOR PREDICTING BIOCONCENTRATION OF WELL‐METABOLIZED COMPOUNDS BY RAINBOW TROUT USING MEASURED RATES OF IN VITRO INTRINSIC CLEARANCE
Models were developed to predict the bioconcentration of well‐metabolized chemicals by rainbow trout. The models employ intrinsic clearance data from in vitro studies with liver S9 fractions or isolated hepatocytes to estimate a liver clearance rate which is extrapolated to a whole‐body biotransformation rate constant (kMET). Estimated kMET values are then used as inputs to a mass‐balance ...
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Influence of a novel radiopacifier (nano-sized strontia particles) on in vitro properties of a PMMA bone cement for use in total joint replacements
In literature reports on strontia-containing PMMA bone cements, the strontia was combined with another material, micron-sized particles were used and in vitro fatigue data are sparse. The present report is on the in vitro characterisation of a strontia-containing PMMA bone cement in which nano-sized strontia particles that were not combined with any other material were used (NANOSRO cement). ...
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