in vivo study Articles
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Tissue explant co‐culture model of the hypothalamic‐pituitary‐gonadal‐liver axis of the fathead minnow (Pimephales promelas) as a predictive tool for endocrine disruption
Endocrine disrupting compounds (EDCs) can impact the reproductive system by interfering with the hypothalamic‐pituitary‐gonadal (HPG) axis. While in vitro testing methods have been developed to screen chemicals for endocrine disruption, extrapolation of in vitro response to in vivo action shows inconsistent accuracy. We describe here our tissue co‐culture of the fathead minnow (Pimephales ...
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Computer Vision-Based Digital Biomarkers Could Enable Faster and More Accurate In Vivo Assessment of Disease
At Recursion, we are working to decode biology and industrialize drug discovery. We have successfully executed over 82 million phenomic experiments and inferred over 179 billion biological relationships in our quest to identify promising novel therapeutic approaches. Now, we are extending our industrialized approach to petabytes of pre-clinical in vivo studies. Digitalizing in vivo studies ...
By Recursion
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Augmentation of anti–tumour activity of cisplatin by pectin nano–conjugates in B–16 mouse model: pharmacokinetics and in–vivo biodistribution of radio–labelled, hydrophilic nano–conjugates
Nanoconjugates have matured from simple devices to multifunctional, biodegradable, non–toxic and non–immunogenic constructs, capable of delivering synergistically functioning drugs in vivo. The present study evaluates the efficacy of drug polymer self folding nano–conjugates of pectin–cisplatin to enhance blood circulating levels of cisplatin. Physical characterisation was done by DLS, zeta ...
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Rational`s Experimental Herpes Vaccine Shows Preclinical Promise
Rational Vaccines said findings from a pilot study showed that RVX201 led to a reduction in recurrent genital lesions when compared to gD2-alum/MPL vaccine in animal studies. Rational's Experimental Herpes Vaccine Shows Preclinical Promise Rational Vaccines said findings from a pilot study showed that RVX201 led to a reduction in recurrent genital lesions when compared to gD2-alum/MPL vaccine ...
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Neutral atom beam technique enhances bioactivity of PEEK
Abstract Polyetheretherketone (PEEK) is currently gaining popularity in orthopedic and spinal applications but has potential drawbacks in use. PEEK is biocompatible, similar in elasticity to bone, and radiolucent; however, it has been shown to be inert and does not integrate well with bone. Recent efforts have focused on increasing the bioactivity of PEEK by modifying the surface to improve the ...
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Cyclin D1 Genetic Heterozygosity Regulates Colonic Epithelial Cell Differentiation and Tumor Number in ApcMin Mice
Constitutive ß-catenin/Tcf activity, the primary transforming events in colorectal carcinoma, occurs through induction of the Wnt pathway or APC gene mutations that cause familial adenomatous polyposis. Mice carrying Apc mutations in their germ line (ApcMin) develop intestinal adenomas. Here, the crossing of ApcMin with cyclin D1–/– mice reduced the intestinal tumor number in animals genetically ...
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Programmed Drug Release of rhBMP-2 for Bone Healing
Summary: Non-union of bone fractures can cause pain and disabilities. Amongst certain fracture types, in specific age groups, the incidence of non-union is over 8% in the United States and over 3.6% of all fractures in the UK. Fracture non-unions have lifelong consequences and a debilitating impact in patients' life. Bone-grafts, allografts and xenograft are currently used to treat bone ...
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Predicted‐no‐effect concentrations for the steroid estrogens estrone, 17β‐estradiol, estriol, and 17α‐ethinylestradiol
The authors derive predicted‐no‐effect concentrations (PNECs) for the steroid estrogens (estrone [E1], 17β‐estradiol [E2], estriol [E3], and 17α‐ethinylestradiol [EE2]) appropriate for use in risk assessment of aquatic organisms. In a previous study, they developed a PNEC of 0.35 ng/L for EE2 from a species sensitivity distribution (SSD) based on all available chronic aquatic toxicity data. The ...
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Endocrine disrupting effect of the UV filter benzophenone‐3 in zebrafish, Danio rerio
The chemical UV filter benzophenone‐3 (BP‐3) is suspected to be an endocrine disruptor based on results from in vitro and in vivo testing. However, studies including endpoints of endocrine adversity are lacking. The present study investigated the potential endocrine disrupting effects of BP‐3 in zebrafish (Danio rerio) in the Fish Sexual Development Test (OECD TG 234) and a 12 day adult male ...
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New Study Findings about Glycoprotein PTGDS
Glycoprotein prostaglandin D2 synthase (PTGDS) is a member of the lipocalcitonin superfamily that plays a dual role in prostaglandin metabolism and lipid transport. PTGDS is involved in various cellular processes including solid tumorigenesis, but its role in tumorigenesis is paradoxical, and the significance of PTGDS in hematologic malignancies is not clear. The aim of this new study was to ...
By CD BioGlyco.
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A Closer Look at Reporter Lentiviral Particles: Tool for Biomedical Research
Introduction For years, scientists have leveraged the brilliant potentialities of genetic science to explore how cells function and interact in living organisms. Among the numerous tools employed are "reporter systems," which use a visible marker to track gene expression, protein interactions, and other vital biological processes. One such system employs reporter lentiviral particles, using the ...
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Key Takeaways from BIO 2022 for AI-enabled Drug Discovery
It was great to be back in person at the 2022 BIO International Convention this year. It came as no surprise to us at Recursion that artificial intelligence (AI) and machine learning (ML) were hot topics at the conference, with more than half a dozen sessions exploring how these technologies are driving digital transformation in biopharma. We heard from leaders who are applying AI at ...
By Recursion
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Idience: developing selective and potent PARP inhibitor IDX-1197 for solid tumors
The first wave of PARP inhibitors has improved outcomes in people with a variety of solid tumors. However, Idience has identified opportunities to improve the efficacy of existing drugs and overcome resistance. The company plans to target homologous recombination repair-proficient patients with a clinical-phase PARP inhibitor that is now available for licensing. Ildong Holdings, a major ...
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Advanced Approaches in Inducible Expression Stable Cell Line Development
Harnessing the power of cell biology, scientists across the globe are perpetually invested in the pursuit of understanding the complexities of cellular mechanisms and their adaptability. One such venture is the creation of Inducible Expression Stable Cell Line (IESCL) development. IESCL signifies a consolidated methodology designed to explore the potentialities of modulated gene expression. This ...
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Accurately Recapitulating Environmental Niches in Vitro Models of the Intestine
Advanced microphysiological systems can replicate aspects of intestinal complexity, such as epithelium self-renewal by stem cells in in vitro crypts or the interactions of microbes and intestinal epithelium mediated by a mucus layer. Availability of such models is key to progress in compound screening, disease modeling, and microbiome research. Defining a Niche A niche is a multidimensional ...
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A hybrid modeling approach for assessing mechanistic models of small molecule partitioning in vivo using a machine learning integrated modeling platform
Prediction of the first-in-human dosing regimens is a critical step in drug development and requires accurate quantitation of drug distribution. Traditional in vivo studies used to characterize clinical candidate’s volume of distribution are error-prone, time and cost intensive and lack reproducibility in clinical settings. The paper demonstrates how a computational platform integrating ...
By VeriSIM Life
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Effect of interferon receptor pathway on CAR-T cell-mediated killing of solid tumors
Researchers at Massachusetts General Hospital (MGH) have found that interferon gamma receptor (IFNgR) signaling is essential for the susceptibility of CAR-T cell immunotherapy to kill malignant gliomas. The same phenomenon has been observed in other solid tumors. The findings may partially explain why liquid and solid tumors respond very differently to CAR-T cell therapy. Chimeric antigen ...
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The Five Factors of Smart Laboratory Success
The Five Factors Research organizations are under pressure to cut costs while maintaining or increasing throughput. To improve their operations, many laboratories are considering investments in data management or support and automation systems like ELNs, LIMS or Robotics/HTS. Whatever the change that is introduced, there are many pitfalls on the way to delivery, roll-out and successful ...
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Increasing the Efficiency of Drug Development with Preclinical Testing Using Human Intestinal Stem Cells
Due to a lack of robust in vivo gut models, animal studies are generally required to evaluate gut toxicity. However, these studies can be lengthy and expensive and may not accurately recapitulate the behavior of the human gastrointestinal tract. Drugs are thus often developed with undesired gut side effects that are not apparent until clinical trials. Organ-on-a-chip models are the closest ...
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LC/MS/MS analysis of metabolites of synthetic Cannabinoids JWH-018 and JWH-073 in Urine
Abstract A liquid chromatographic method was developed to resolve a comprehensive set of metabolites of JWH-018 and JWH-073. In addition to the chromatographic analysis method, an extraction method was developed to recover a broad range of synthetic cannabinoid metabolites, including carboxylic acid metabolites that are not traditionally recovered using a high pH liquid/liquid extraction. The ...
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