in vivo system Articles
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Tissue explant co‐culture model of the hypothalamic‐pituitary‐gonadal‐liver axis of the fathead minnow (Pimephales promelas) as a predictive tool for endocrine disruption
Endocrine disrupting compounds (EDCs) can impact the reproductive system by interfering with the hypothalamic‐pituitary‐gonadal (HPG) axis. While in vitro testing methods have been developed to screen chemicals for endocrine disruption, extrapolation of in vitro response to in vivo action shows inconsistent accuracy. We describe here our tissue co‐culture of the fathead minnow (Pimephales ...
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High-throughput identification and validation of in situ-expressed genes of lactococcus lactis
Understanding the functional response of bacteria to their natural environment is one of the current challenges in microbiology. Over the past decades several techniques have been developed to study gene expression in complex natural habitats. Most of these methods, however, are laborious, and validation of results under in situ conditions is cumbersome. Here we report the improvement of the ...
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Three scientists won the Wolff Chemistry Award 2022
Recently, the Wolf Foundation published a list of Wolf Chemistry Award winners in 2022, with honors from Professor Bonnie L. Bassler at Princeton University, Professor Carolyn R. Bertozzi at Stanford University, and Professor Benjamin F. Cravatt III at the Scripps Institute. The press release points out that these scientists have made pioneering contributions to understanding the chemistry behind ...
By Profacgen
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Scientists use lipid nanoparticles to precisely target gene-editing tools to the lung
Safe and effective systemic delivery of mRNA to specific organs and cells in vivo remains a major challenge in the development of mRNA-based therapies. Currently, the application of mRNA-based technologies is largely limited to liver diseases because of the lack of specific and effective extrahepatic systemic mRNA delivery systems in vivo. Now, a team of researchers has fine-tuned the existing ...
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Introduction to AAV as a Gene Therapy Vector, Part 2
What are AAVs? and what makes them good candidates for gene therapy? Introduction In the first part of our three part series introducing AAV as a gene therapy vector, we talked about basic AAV vector biology. In this post, we’re going to take a step back to answer the question of “Why AAV?” and look at some opportunities in the AAV engineering space. Why AAV? Viral vectors ...
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A hybrid modeling approach for assessing mechanistic models of small molecule partitioning in vivo using a machine learning integrated modeling platform
Prediction of the first-in-human dosing regimens is a critical step in drug development and requires accurate quantitation of drug distribution. Traditional in vivo studies used to characterize clinical candidate’s volume of distribution are error-prone, time and cost intensive and lack reproducibility in clinical settings. The paper demonstrates how a computational platform integrating ...
By VeriSIM Life
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Accurately Recapitulating Environmental Niches in Vitro Models of the Intestine
Advanced microphysiological systems can replicate aspects of intestinal complexity, such as epithelium self-renewal by stem cells in in vitro crypts or the interactions of microbes and intestinal epithelium mediated by a mucus layer. Availability of such models is key to progress in compound screening, disease modeling, and microbiome research. Defining a Niche A niche is a multidimensional ...
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The Power Of RepliGut in Vitro Intestinal Stem Cell Models
Both current in vitro and in vivo models used in the development of candidate therapies for the treatment of intestinal diseases have severe limitations. RepliGut®, an in vitro human intestinal epithelial stem cell model, addresses many of these issues and has significant potential to reduce the time and cost of drug development. This model may even help to identify optimal therapies for ...
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