in vivo use Articles
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Transcriptional Regulation of xyn2 in Hypocrea jecorina
The xylanase system of the filamentous fungus Hypocrea jecorina (Trichoderma reesei) consists of two specific xylanases, Xyn1 and Xyn2, which are simultaneously expressed during growth on xylan but respond differentially to low-molecular-weight inducers. Using in vivo footprinting analysis of xylan-induced and noninduced mycelia, we detected two adjacent nucleotide sequences (5'-AGAA-3' on the ...
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Predicted‐no‐effect concentrations for the steroid estrogens estrone, 17β‐estradiol, estriol, and 17α‐ethinylestradiol
The authors derive predicted‐no‐effect concentrations (PNECs) for the steroid estrogens (estrone [E1], 17β‐estradiol [E2], estriol [E3], and 17α‐ethinylestradiol [EE2]) appropriate for use in risk assessment of aquatic organisms. In a previous study, they developed a PNEC of 0.35 ng/L for EE2 from a species sensitivity distribution (SSD) based on all available chronic aquatic toxicity data. The ...
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CYP‐mediated warfarin metabolic ability is not a critical determinant of warfarin sensitivity in avian species; in vitro assays in several birds and in vivo assays in chicken
Coumarin derivative anticoagulant rodenticides used for rodent control are posing a serious risk to wild bird populations. For warfarin (WF) a classic coumarin derivative, chicken have a high LD50 while mammalian species generally have much lower LD50. Large interspecies differences in sensitivity to WF are to be expected. We previously reported substantial differences in WF metabolism among ...
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Evaluation of the scientific underpinnings for identifying estrogenic chemicals in nonmammalian taxa using mammalian test systems
The US Environmental Protection Agency has responsibility for assessing endocrine activity of more than 10 000 chemicals, a task that cannot reasonably be achieved solely through use of available mammalian and nonmammalian in vivo screening assays. Hence, it has been proposed that chemicals be prioritized for in vivo testing using data from in vitro high‐throughput assays for specific ...
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Neutral atom beam technique enhances bioactivity of PEEK
Abstract Polyetheretherketone (PEEK) is currently gaining popularity in orthopedic and spinal applications but has potential drawbacks in use. PEEK is biocompatible, similar in elasticity to bone, and radiolucent; however, it has been shown to be inert and does not integrate well with bone. Recent efforts have focused on increasing the bioactivity of PEEK by modifying the surface to improve the ...
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A hybrid modeling approach for assessing mechanistic models of small molecule partitioning in vivo using a machine learning integrated modeling platform
Prediction of the first-in-human dosing regimens is a critical step in drug development and requires accurate quantitation of drug distribution. Traditional in vivo studies used to characterize clinical candidate’s volume of distribution are error-prone, time and cost intensive and lack reproducibility in clinical settings. The paper demonstrates how a computational platform integrating ...
By VeriSIM Life
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Recombinant Cyno NKG2A & CD94 Heterodimer
Product name NKG2A and CD94 heterodimer (KLRC1), recombinant protein Full Product Name Recombinant human NKG2A and CD94 heterodimeric protein (N-8His and N-DYKDDDDK) Product synonym names Heterodimer NKG2A and CD94; Heterodimer KLRC1 and CD94; CD159A and KLRD1 heterodimer Product gene name Recombinant protein KLRC1 3D structure ModBase 3D Structure for P26715 Host: Human cells Purity ...
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Neuronal processes that drive behavioral motivation have been found, according to Science
"A neuronal mechanism for motivational control of behavior" published in Science by Friedrich Miescher Institute for Biomedical Research, University of Basel. Almost everything you do in your daily life is geared toward achieving a specific goal. The brain has the ability to maintain a motivated state in order to lead behaviors toward desired results. Deep brain calcium imaging, ...
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Efficacy of antimicrobial polymer coatings in an animal model of bacterial infection associated with foreign body implants
Abstract Objectives: To assess support discs, comprising polyethylene terephthalate (PET), coated with different polymer/levofloxacin combinations for antimicrobial activity in an animal model of infection, in order to explore the use of specific polymer coatings incorporating levofloxacin as a means of reducing device-related infections. Methods: Aliphatic polyester-polyurethanes ...
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The Power Of RepliGut in Vitro Intestinal Stem Cell Models
Both current in vitro and in vivo models used in the development of candidate therapies for the treatment of intestinal diseases have severe limitations. RepliGut®, an in vitro human intestinal epithelial stem cell model, addresses many of these issues and has significant potential to reduce the time and cost of drug development. This model may even help to identify optimal therapies for ...
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Label-based Protein Quantification Technology: iTRAQ, TMT, SILAC
Changes in the number of proteomes in a cell have a big effect on many aspects of life. For example, the occurrence and development of many diseases are often accompanied by abnormal expression of certain proteins. Quantitative proteomics is the accurate quantification and identification of all proteins expressed in a genome or all proteins in a complex mixed system. At present, quantitative ...
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A Novel, High-Throughput Platform to Characterize Novel IBD Therapeutics
Tumor Necrosis Factor Alpha (TNF-a) is a potent cytokine that is over-expressed by cells during chronic intestinal inflammation. Furthermore, TNF-a represents the most validated clinical target for ulcerative colitis (UC) and inflammatory bowel diseases (IBD) with multiple emerging neutralizing/inflammation-reducing therapeutics targeting this cytokine for inactivation. TNF-a activation of ...
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Innovative technology of engineering magnetic DNA nanoparticles for gene therapy
The present research study is a novel innovation materials technology to engineer magnetic DNA nanoparticles for gene therapy and to enhance the localisation and gene expression of a plasmid DNA via magnetic DNA nanoparticles. Spermine (Sm) was chemically introduced to the hydroxyl groups of dextran, a polysaccharide, to obtain cationised dextran (dextran-Sm). When Fe2+ solution was added to the ...
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In ovo exposure to nonylphenol and bisphenol A resulted in dose‐independent feminization of male gonads in Japanese quail (Coturnix japonica) embryos
Sex reversal effects of nonylphenol and bisphenol A on the gonads in F1(AWE × WE) Japanese quail (Coturnix japonica) embryos were investigated using an in vivo screening model developed previously. The F1 (AWE × WE) Japanese quail are a useful avian model because sex differentiation is confirmed by the plumage color before hatching, ruled by a criss‐cross inheritance. The nonylphenol at 200, ...
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Biomedical applications of iron oxide nanostructures
Iron oxide nanostructures including nanoparticles and nanorods have been widely used for numerous in vivo and vitro applications such as magnetic resonance imaging contrast enhancement, tissue repair and drug delivery. All these biomedical and bioengineering applications require that these nanostructures have high magnetisation values and size smaller then 100 nm with overall narrow particle size ...
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Perfluorooctane sulfonate (PFOS) affects hormone receptor activity, steroidogenesis, and expression of endocrine‐related genes in vitro and in vivo
Perfluorooctane sulfonate (PFOS) is a widespread and persistent chemical in the environment. We investigated the endocrine‐disrupting effects of PFOS using a combination of in vitro and in vivo assays. Reporter gene assays were used to detect receptor‐mediated (anti‐)estrogenic, (anti‐)androgenic, and (anti‐)thyroid hormone activities. The effect of PFOS on steroidogenesis was assessed both at ...
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Alleviation of DSS-induced colitis via Lactobacillus acidophilus treatment in mice
Abstract Gut microbiota play a major role in host physiology and immunity. Inflammatory bowel diseases (IBDs), the important immune-related diseases, can occur through immune system malfunction originating due to dysregulation of the gut microbiota. The aim of this study was to investigate the capabilities and mechanisms of Lactobacillus acidophilus (L. acidophilus) KBL402 and KBL409 treatment ...
By KoBioLabs
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Cell-Penetrating Peptides: Features, Mechanisms, Applications
Cell-penetrating peptides (CPPs) refer to water-soluble, partly hydrophobic, and/or polybasic short peptides that are fewer than 30 amino acids in size. Different types of CPPs like chimeric peptides and hydrophobic peptides have different lengths, charges, solubility, and hydrophobicity. At current, cell-penetrating peptides are often classified in line with their characteristics, including ...
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Development of Gene Therapy Viral Vectors for Rare Diseases
In many cases, gene therapy requires a vector to deliver the gene therapy drug to the target cell. Viral vectors have been one of the most widely studied vectors due to their outstanding transduction efficiency and other significant advantages. Viral vector-based gene therapy has now achieved good clinical results. More than a dozen viral gene therapy products have been approved for the treatment ...
By Protheragen
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How Much Do You Know about Bacteriocin?
In recent years, the increase in the number of multi-drug resistant pathogens and food safety have become serious global problems, and it is increasingly important to find or develop a new generation of antibacterial drugs or preservatives. Scientists have discovered that bacteria-produced bacteriocins can control clinically relevant susceptible and resistant bacteria, and purified bacteriocins ...
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