mrna Articles
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MKP-1 mRNA stabilization and translational control by RNA-binding proteins HuR and nf90
The mitogen-activated protein (MAP) kinase phosphatase 1 (MKP-1) plays a major role in dephosphorylating and thereby inactivating the MAP kinases extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK), and p38. Here, we examine the posttranscriptional events underlying the robust MKP-1 induction by oxidants in HeLa cells. H2O2 treatment potently stabilized the MKP-1 mRNA and ...
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Codon optimization increases steady-state mrna levels in aspergillus oryzae heterologous gene expression
We investigated the effect of codon optimization on the expression levels of heterologous proteins in Aspergillus oryzae, using the mite allergen Der f 7 as a model protein. A codon-optimized Der f 7 gene was synthesized according to the frequency of codon usage in A. oryzae by recursive PCR. Both native and optimized Der f 7 genes were expressed under the control of a ...
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Chaperone hsp27, a novel subunit of auf1 protein complexes, functions in au-rich element-mediated mrna decay
Controlled, transient cytokine production by monocytes depends heavily upon rapid mRNA degradation, conferred by 3' untranslated region-localized AU-rich elements (AREs) that associate with RNA-binding proteins. The ARE-binding protein AUF1 forms a complex with cap-dependent translation initiation factors and heat shock proteins to attract the mRNA degradation machinery. We refer to this protein ...
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How Moderna is Building a Digital Biotech
At Moderna, building a digital biotech is a foundational component of who we are and is a key enabler of our mission to create a new class of mRNA medicines for patients. Incorporating digital into everything we do is mission-critical to us. Read about our digitization strategy new white paper, which includes the ability of our digital infrastructure, built from ground up, to enable ...
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Development of a molecular biomarker for detecting intersex after exposure of male medaka fish to synthetic estrogen
Although intersex of fish species has been widely reported in aquatic environments, there is no appropriate biomarker of the effects to assess the occurrence of intersex. In the present study, mRNA expression profiles of 14 genes, which are either involved in ovary development and maintenance or have relatively high mRNA transcription levels in ovarian gene expression profiles in fish species, ...
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Exposure to the pesticide linuron affects androgen‐dependent gene expression in the three‐spined stickleback (Gasterosteus aculeatus)
Previous research demonstrated that exposure to exogenous androgens and effluents with androgenic activity can induce spiggin mRNA production in the kidney of the three‐spined stickleback (Gasterosteus aculeatus). In the present study, we determine whether a short‐term exposure to a known antiandrogenic pesticide, linuron (LN), suppresses spiggin mRNA in male stickleback and in androgenized ...
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Modulations in androgen and estrogen mediating genes and testicular response in male goldfish exposed to bisphenol A
Adverse effects of bisphenol A (BPA) on male reproductive physiology were studied in male goldfish (Carassius auratus) exposed to nominal environmentally relevant concentrations (0.2 and 20 µg/L) for up to 90 d. Transcriptions of various reproductive genes were measured in brain, liver, and testis to investigate the BPA modes of action. Volume, density, total number, and motility and velocity of ...
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Molecular identification and function of cis- and trans-acting determinants for peta transcript stability in chlamydomonas reinhardtii chloroplasts
In organelles, the posttranscriptional steps of gene expression are tightly controlled by nucleus-encoded factors, most often acting in a gene-specific manner. Despite the molecular identification of a growing number of factors, their mode of action remains largely unknown. In the green alga Chlamydomonas reinhardtii, expression of the chloroplast petA gene, which codes for cytochrome f, depends ...
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Cadmium inhibits the vitellogenesis of freshwater crab Sinopotamon henanense
Cadmium (Cd) may pose risks to freshwater organisms, including crabs that live at the interface of sediments and water column all year round. One of the major changes that occur during oocyte maturation of crabs is the production of vitellin (Vn). In the present study, we investigated the effects of Cd on oocyte size, Vn level, and vitellogenin (Vg) mRNA expression in the ovary of the ...
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Regulation of abcg2 expression at the 3` untranslated region of its mrna through modulation of transcript stability and protein translation by a putative microrna in the s1 colon cancer cell line
ABCG2 is recognized as an important efflux transporter in clinical pharmacology and is potentially important in resistance to chemotherapeutic drugs. To identify epigenetic mechanisms regulating ABCG2 mRNA expression at its 3' untranslated region (3'UTR), we performed 3' rapid amplification of cDNA ends with the S1 parental colon cancer cell line and its drug-resistant ABCG2-overexpressing ...
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mRNA Delivery System
RNA is a hydrophilic, negatively charged macromolecule, and its ability to autonomously transmembrane is limited. Therefore, how to efficiently deliver mRNA to the cytoplasm and play a corresponding role is one of the key issues that limit the application of RNA therapy. The wide range of applications of therapeutic mRNA requires efficient and safe delivery methods. An ideal delivery system ...
By BOC Sciences
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The Significance of Raw Enzymes in the Success of mRNA Vaccines
In the ever-evolving landscape of global healthcare, mRNA vaccines have emerged as an excellent tool in the fight against infectious diseases. This article explores the intricate and indispensable role that raw enzymes play in the manufacturing of mRNA vaccines, delving deep into their significance and impact on vaccine production. As we venture into this fascinating world, we will gain a ...
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Bespoke mRNA Synthesis Service: Pioneering customizable RNA solutions in the Sphere of Biotechnology
In the ever-evolving realm of biotechnology, the astonishing advancements in mRNA (messenger RNAs) synthesis are poised to revolutionize various aspects of biological research. This article delineates the eclectic vistas opened up by custom mRNA synthesis services that provide bespoke, high-quality solutions for researchers globally. mRNA synthesis, an integral element of our genetic machinery, ...
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Self-amplifying RNA as a tool to tackle emerging flavivirus outbreaks: lessons learned from the COVID-19 pandemic
Background Outbreaks of multiple Flaviviruses have afflicted humankind for centuries due to their rapid spread predominantly by arthropod vectors. Global warming has caused epidemics to emerge in previously unaffected regions. The SARS-CoV-2 outbreak and subsequent race in vaccine development can provide strategies to the scientific community to avoid a flavivirus pandemic. The COVID-19 battle ...
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An Overview of PseudoUridine Modification in RNAs
It is an efficient way to regulate molecular function by applying specific chemical modifications on biological molecules. This article highlights the current heated pseudoUridine modification in RNA, which suggests new possibilities for novel drug or vaccine discovery. An Introduction to PseudoUridines PseudoUridine (Ψ) is a post-transcriptional RNA modification that alters RNA-RNA and ...
By BOC Sciences
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Norfloxacin drug induces reproductive toxicity and alters androgen receptor gene expression in testes and cloacal gland of male Japanese quail (coturnix japonica)
Attempts have been made to investigate the reproductive toxicity of norfloxacin drug in Japanese quail. Male quail were administered with norfloxacin @ 20 mg/kg body weight for 14 days. Then reproductive functions and androgen receptor (AR) gene expression was examined in treated and control birds. The results of the present study indicated that fertility, cloacal gland area, sperm ...
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Scientists use lipid nanoparticles to precisely target gene-editing tools to the lung
Safe and effective systemic delivery of mRNA to specific organs and cells in vivo remains a major challenge in the development of mRNA-based therapies. Currently, the application of mRNA-based technologies is largely limited to liver diseases because of the lack of specific and effective extrahepatic systemic mRNA delivery systems in vivo. Now, a team of researchers has fine-tuned the existing ...
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RNA Splicing and How to Target It
What Is RNA Splicing? RNA splicing is a post-transcriptional modification process that occurs in eukaryotic cells. When a gene is transcribed, the initial RNA product is called pre-mRNA, which contains both coding regions (exons) and non-coding regions (introns). RNA splicing removes the introns from the pre-mRNA molecule and joins together the exons to form a mature mRNA molecule that can be ...
By CD Genomics
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The RNA binding protein hnRNP Q modulates the utilization of exon 7 in the survival motor neuron 2 (SMN2) gene
Spinal muscular atrophy (SMA) is a recessive neuromuscular disorder caused by the homozygous loss of the SMN1 gene. The human SMN2 gene has a C-to-T transition at position +6 of exon 7 and thus produces exon 7-skipping mRNAs. However, we observed an unexpectedly high level of exon 7-containing SMN2 transcripts as well as SMN protein in testis of smn–/– SMN2 transgenic mice. Using affinity ...
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Feedback regulation between zipcode binding protein 1 and β-catenin mrnas in breast cancer cells
ZBP1 (zipcode binding protein 1) is an RNA-binding protein involved in many posttranscriptional processes, such as RNA localization, RNA stability, and translational control. ZBP1 is abundantly expressed in embryonic development, but its expression is silenced in most adult tissues. Reactivation of the ZBP1 gene has been reported in various human tumors. In this study, we identified a detailed ...
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