PCR assay Articles
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Mic and Myra Prove Flexible and Reliable in Regional Mobile Labs
Mic qpcr cycler and Myra liquid handler have been put through their paces in COVID-19-testing field trials in regional Western Australia and found to be noticeably robust, rapidly deployable and flexible. These results have been outlined in a recent paper, Development, deployment and in-field demonstration of mobile corona virus SARS-CoV-2 Nucleic acid amplification test, published by Paton, ...
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Using molecular biomarkers and traditional morphometric measurements to assess the health of slimy sculpin (Cottus cognatus) from streams with elevated selenium in north‐eastern British Columbia
Canadian fish‐based environmental effects monitoring programs use individual and population level endpoints to assess aquatic health. Impacts of coal mining and selenium (Se) exposure were assessed in slimy sculpin (Cottus cognatus) from reference streams located both inside and outside of a coal zone, and from one stream with a history of coal mining, using traditional environmental effects ...
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PCR assay targeting virulence genes of
Helicobacter pylori isolated from drinking water and clinical samples in Lahore metropolitan, PakistanHelicobacter pylorus is considered for chronic gastritis, gastric ulcers and adenocarcinoma and its high infection rate is observed in overcrowded and lower socioeconomic groups in developing countries. This study was designed to identify the role of drinking water in the transmission and prevalence of H. pylori (HP). Selective HP medium was developed for enrichment and presumptive identification ...
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Occurrence of antibiotic resistance genes in reclaimed water and river water in the Werribee Basin, Australia
The purpose of this study was to investigate the occurrence of antibiotic resistance genes (ARGs) in water used for irrigation in the Werribee River Basin, Australia, including river water and reclaimed effluent water (reclaimed water). Samples of reclaimed water, collected over a one-year period, were screened for the occurrence of ARGs using PCR detection assays. The presence of ARGs in the ...
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The Molecular Microbiology of SARS-CoV-2: Detection Methods and Infection Mechanism
SARS-CoV-2 is a newly discovered human coronavirus group (HCoV), which are single-stranded RNA viruses belonging to the family of Coronaviridae. A range of respiratory diseases have been induced by some of these pathogens in the past. SARS-CoV-1 infected more than 8,000 individuals around the world, for example. Human coronavirus linked to Middle East respiratory syndrome (MERS-CoV) also occurred ...
By CD Genomics
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Characterization of an Sf-rhabdovirus-negative Spodoptera frugiperda cell line as an alternative host for recombinant protein production in the baculovirus-insect cell system
Abstract Cell lines derived from the fall armyworm, Spodoptera frugiperda (Sf), are widely used as hosts for recombinant protein production in the baculovirus-insect cell system (BICS). However, it was recently discovered that these cell lines are contaminated with a virus, now known as Sf-rhabdovirus [1]. The detection of this adventitious agent raised a potential safety issue that could ...
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