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Primary Antibodie Articles & Analysis
4 articles found
But we've never had a set "plan" to find the most suitable antibody. In this issue, the editor shares a 10-step "plan" for antibody selection.Determine the Name of the Target ProteinFirst, you need to be clear about what protein you need to detect in order to select the corresponding antibody. ...
Expasy and NCBI BLAST can be used to compare the homology of proteins of different species. Selection of primary host species When using a conjugated secondary antibody combined with a primary antibody without a conjugate, the species selection of the host animal of the primary antibody is more ...
The samples were analyzed by Wes™ using a total protein concentration of 0.25–0.5 µg/µL for each sample, the Jess/Wes Separation kit (12–230 kDa), primary antibodies against ERK1/2, p44/42 MAPK (137F5) Rabbit mAb (Cell Signaling Technology, Danvers, MA, USA, Cat. ...
In the study, the researchers propose a new method that mainly involves extensive screening of monoclonal antibodies against primary human tumor samples with the aim of identifying cancer-specific conformational epitopes throughout the protein that cannot be identified by transcriptome or proteome analysis. ...