protein expression Articles
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Analysis of a novel insect cell culture medium-based growth medium for bartonella species
Human- and animal-pathogenic Bartonella species are fastidious and slow-growing bacteria difficult to isolate and cultivate. We describe a novel, easy-to-prepare liquid medium for the fast and reliable growth of several Bartonella spp. that does not affect bacterial protein expression patterns or interactions with host ...
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Using a kinetic model that considers cell segregation to optimize hEGF expression in fed-batch cultures of recombinant Escherichia coli
Growth inhibition of recombinant Escherichia coli during the expression of human epidermal growth factor was observed. The recombinant cells could be segregated into three populations based on their cell division and plasmid maintenance abilities: dividing and plasmid-bearing cells, dividing and plasmid-free cells, and viable-but-non-culturable (VBNC) cells. Fed-batch fermentations were performed ...
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Low doses of γ–radiation induce consistent protein expression changes in human leukocytes
Twenty percent of cancer patients experience adverse effects after radiotherapy. The therapeutic doses are adjusted to the most sensitive individuals, resulting in a suboptimal dose for many patients. At present there is no screening system available to predict individual radiosensitivity. The main aim of this study is to investigate differences in protein expression pathways induced by low doses ...
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Selection against puma gene expression in myc-driven b-cell lymphomagenesis
The p53 tumor suppressor pathway limits oncogenesis by inducing cell cycle arrest or apoptosis. A key p53 target gene is PUMA, which encodes a BH3-only proapoptotic protein. Here we demonstrate that Puma deletion in the Eµ-Myc mouse model of Burkitt lymphoma accelerates lymphomagenesis and that 75% of Eµ-Myc lymphomas naturally select against Puma protein expression. Furthermore, approximately ...
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Upregulation of annexin a1 expression by butyrate in human colon adenocarcinoma cells: role of p53, nf-y, and p38 mitogen-activated protein kinase
Annexin A1 is a member of a phospholipid and calcium binding family of proteins; it is involved in anti-inflammation and in the regulation of differentiation, proliferation, and apoptosis. Here, we show the existence of a functional binding site for the tumor suppressor p53 near the proximal CCAAT box and the fact that the basal expression of annexin A1 in human colon adenocarcinoma cells is ...
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RNA-targeted Gene Therapy
In recent years, various nucleic acid-based therapies (NBT) have emerged as effective and specific activators of endogenous gene expression. Unlike gene therapy approaches supplementing gene expression, RNA-targeted therapy enhances protein production by selectively modulating cellular mechanisms mediated by endogenous RNA, such as transcription, splicing, translation, mRNA stability, and ...
By BOC Sciences
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The developmental neurotoxicity of PBDEs: Effect of DE‐71 on dopamine in zebrafish larvae
The potential neurotoxicity of polybrominated diphenyl ethers (PBDEs) is still a great concern. In the present study, we investigated whether exposure to PBDEs could affect the neurotransmitter system and cause developmental neurotoxicity in zebrafish. Two hours post fertilization (hpf) zebrafish embryos were exposed to different concentrations of the PBDE mixture DE‐71 (0‐100 µg/L). The ...
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The Notable Breakthroughs in Drug Discovery with SILAC Technique
Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) is a powerful technique that allows for the quantification and characterization of proteins in cells and organisms. It involves the substitution of specific amino acids with their isotopically labeled counterparts in cell culture media. This labeling enables researchers to track and compare protein expression levels, protein-protein ...
By BOC Sciences
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Acute exposure to DE‐71: Effects on locomotor behavior and developmental neurotoxicity in zebrafish larvae
The aim of the present study was to investigate the acute developmental neurotoxicity of polybrominated diphenyl ethers (PBDEs) in zebrafish larvae. From 2 to 120 h postfertilization Zebrafish embryos at were exposed to DE‐71 (0, 31.0, 68.7, and 227.6 µg/L). We studied the locomotor behavior of larvae, involvement of the cholinergic system, and selected gene and protein expressions in the central ...
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Characterization of three classes of membrane proteins involved in fungal azole resistance by functional hyperexpression in saccharomyces cerevisiae
The study of eukaryotic membrane proteins has been hampered by a paucity of systems that achieve consistent high-level functional protein expression. We report the use of a modified membrane protein hyperexpression system to characterize three classes of fungal membrane proteins (ABC transporters Pdr5p, CaCdr1p, CaCdr2p, CgCdr1p, CgPdh1p, CkAbc1p, and CneMdr1p, the major facilitator superfamily ...
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Functionalised silica nanoparticles stable in serum–containing medium efficiently deliver siRNA targeting HIV–1 co–receptor CXCR4 in mammalian cells
The development of non–viral DNA delivery systems using nanomaterials has attracted much research interest for its potential in biomedicine. However, for these new nanocarriers to be successfully used in therapeutic applications they have to overcome many barriers. Here, we report the development and characterisation of polyethyleneimine–modified tetramethylrhodamine–doped silica nanoparticles as ...
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Adventitious viruses in insect cell lines used for recombinant protein expression
Abstract Insect cells are widely used for recombinant protein expression, typically as hosts for recombinant baculovirus vectors, but also for plasmid-mediated transient transfection or stable genetic transformation. Insect cells are used to express proteins for research, as well as to manufacture biologicals for human and veterinary medicine. Recently, several insect cell lines used for ...
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Label-based Protein Quantification Technology: iTRAQ, TMT, SILAC
Changes in the number of proteomes in a cell have a big effect on many aspects of life. For example, the occurrence and development of many diseases are often accompanied by abnormal expression of certain proteins. Quantitative proteomics is the accurate quantification and identification of all proteins expressed in a genome or all proteins in a complex mixed system. At present, quantitative ...
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Transcriptional expression of H2B, CTP synthase and PLK3 genes in whole blood exposed to 60Co gamma radiation
Ionising radiation induces complex molecular responses in human cells resulting in changes at mRNA and protein expression. Limited data is available on the transcriptional status of functional genes in response to ionising radiation using peripheral blood mononuclear cells (PBMCs). In the present study, transcriptional profiles of Histone 2B, CTP synthase and PLK3 were studied. Blood samples were ...
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The scientific paper about PPI-FIT technology
And its first validation is now published on Nature Communications Biology! Spagnolli, G., Massignan, T., Astolfi, A. et l. Pharmacological inactivation of the prion protein by targeting a folding intermediate. Commun Biol 4, 62 2021). Recent computational advancements in the simulation of biochemical processes allow investigating the mechanisms involved in protein regulation with realistic ...
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Fabrication Of In-Vitro Phenotypically Stable Engineered Articular Cartilage
Researchers from IRCCS Istituto Ortopedico Rizzoli and Indian Institute of Technology, Delhi (IIT) used their REGENHU bioprinters to fabricate an in vitro phenotypically stable engineered articular cartilage by embedding human bone marrow-derived stromal cells (hMSCs) in a cell-laden silk fibroin–gelatin (SF-G) bioink. The bioprinted constructs allowed the in vitro study of chondrogenic ...
By Regenhu
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Exploring the Magic of Tn5 Transposase
In the realm of molecular biology and genetic engineering, Tn5 Transposase is a powerful tool that has revolutionized the way we manipulate and study DNA. It's like molecular scissors and glue combined, allowing us to precisely cut, move, and insert DNA sequences. In this article, we will unravel the wonders of Tn5 Transposase, its applications, and why it's an indispensable asset for researchers ...
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Acute exposure to DE‐71 causes alterations in visual behavior in zebrafish larvae
Polybrominated diphenyl ethers (PBDEs) cause neurobehavioral toxicity, but their effects on visual behavior remain unknown. In the present study, the impact of PBDEs on visual behavior was examined using optokinetic responses and phototaxis in zebrafish larvae. Zebrafish embryos were exposed to DE‐71 at concentrations of 0, 0.32, 3.58 and 31.0 µg/L until 15 days post‐fertilization. We then ...
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Transcriptional expression of H2B, CTP synthase and PLK3 genes in whole blood exposed to 60Co gamma radiation
Ionising radiation induces complex molecular responses in human cells resulting in changes at mRNA and protein expression. Limited data is available on the transcriptional status of functional genes in response to ionising radiation using peripheral blood mononuclear cells (PBMCs). In the present study, transcriptional profiles of Histone 2B, CTP synthase and PLK3 were studied. Blood samples were ...
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Engineering of a human vaginal lactobacillus strain for surface expression of two-domain cd4 molecules
Women are at significant risk of heterosexually transmitted human immunodeficiency virus (HIV) infection, with the mucosal epithelium of the cervix and vagina serving as a major portal of entry. The cervicovaginal mucosa naturally harbors dynamic microflora composed predominantly of lactobacilli, which may be genetically modified to serve as a more efficient protective barrier against the ...
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