real-time PCR Articles
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SARS-CoV-2 Multi-FAST
We have great news to share: we launched Schep SARS-CoV-2 Multi-FAST, a new PCR diagnostic test for the rapid detection of SARS-CoV-2, now in multiplex and in a single step format! Simple detection, by reverse transcription and Real Time PCR. It consists of a single Master Mix, ready to use, containing the enzymes, primers and probes, specific for 3 viral genes: Envelope (E), Nucleocapsid (N) ...
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Evaluation of real-time PCR for quantitative detection of
Escherichia coli in beach waterThe current investigation evaluated the use of real-time polymerase chain reaction (PCR) for quantitative detection of Escherichia coli in marine beach water. Densities of E. coli in 263 beach water samples collected from 13 bathing beaches in Hong Kong between November 2008 and December 2009 were determined using both real-time PCR and culture-based methods. Regression analysis showed that ...
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Multiplex fast real-time pcr for quantitative detection and identification of cos- and pac-type streptococcus thermophilus bacteriophages
The fermentation of milk by Streptococcus thermophilus is a widespread industrial process that is susceptible to bacteriophage attack. In this work, a preventive fast real-time PCR method for the detection, quantification, and identification of types of S. thermophilus phages in 30 min is ...
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Choosing a Real-Time PCR Cycler that works for Your Lab
A real-time PCR cycler can be a significant investment for a lab, and if you’re responsible for the purchase decision you’ll want to do your due diligence and make a future-proof choice. So where do you begin your search? ...
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Exopolysaccharides produced by intestinal bifidobacterium strains act as fermentable substrates for human intestinal bacteria
Eleven exopolysaccharides (EPS) isolated from different human intestinal Bifidobacterium strains were tested in fecal slurry batch cultures and compared with glucose and the prebiotic inulin for their abilities to act as fermentable substrates for intestinal bacteria. During incubation, the increases in levels of short-chain fatty acids (SCFA) were considerably more pronounced in cultures with ...
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Biocientifica SA develops a new high sensitivity RT-PCR kit for SARS-CoV-2
The Argentinian National Administration of Drugs, Food and Medical Technology (ANMAT) approved New kitfor the qualitative detection of the SARS-CoV-2 virus based on Real-Time RT-PCR technology. The Argentinian National Administration of Drugs, Food and Medical Technology (ANMAT) approved Schep SARS-CoV-2 RT-PCR Duo Biocientfica SA, an Argentinian biotechnology company developed this sensitive in ...
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Using molecular biomarkers and traditional morphometric measurements to assess the health of slimy sculpin (Cottus cognatus) from streams with elevated selenium in north‐eastern British Columbia
Canadian fish‐based environmental effects monitoring programs use individual and population level endpoints to assess aquatic health. Impacts of coal mining and selenium (Se) exposure were assessed in slimy sculpin (Cottus cognatus) from reference streams located both inside and outside of a coal zone, and from one stream with a history of coal mining, using traditional environmental effects ...
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Sequencing-Based Methods and Techniques for Microbial Identification
Introduction to Microbial Identification Microbiology research revolves around the detection and identification of microorganisms. It can be used in a variety of fields, including the environment, industry, and medicine. Microbial detection and identification techniques have evolved over time, from conventional methods that are based heavily on culture, morphology, physiology, pathology, and ...
By CD Genomics
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Transcriptional expression of H2B, CTP synthase and PLK3 genes in whole blood exposed to 60Co gamma radiation
Ionising radiation induces complex molecular responses in human cells resulting in changes at mRNA and protein expression. Limited data is available on the transcriptional status of functional genes in response to ionising radiation using peripheral blood mononuclear cells (PBMCs). In the present study, transcriptional profiles of Histone 2B, CTP synthase and PLK3 were studied. Blood samples were ...
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Transcriptional expression of H2B, CTP synthase and PLK3 genes in whole blood exposed to 60Co gamma radiation
Ionising radiation induces complex molecular responses in human cells resulting in changes at mRNA and protein expression. Limited data is available on the transcriptional status of functional genes in response to ionising radiation using peripheral blood mononuclear cells (PBMCs). In the present study, transcriptional profiles of Histone 2B, CTP synthase and PLK3 were studied. Blood samples were ...
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Understanding Residual DNA Testing and Methods for Detecting Residual DNA
Most biological agents enter the body directly without passing through the gastrointestinal tract, so in addition to biological activity, relevant authorities have very strict requirements on impurities in drugs. Among them, the host cells residual DNA has always been the focus of regulatory agencies because of its special potential safety risks. Although recombinant protein drugs, antibody ...
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Administration of Lactobacillus fermentum KBL375 Causes Taxonomic and Functional Changes in Gut Microbiota Leading to Improvement of Atopic Dermatitis
Abstract Gut microbiota play an important role in immune responses and energy metabolism. In this study, we evaluated whether administration of Lactobacillus fermentum (L. fermentum) KBL375 isolated from healthy Korean feces improves the atopic dermatitis using the house dust mite (Dermatophagoides farinae)-induced atopic dermatitis (AD) mouse model. Administration of L. fermentum KBL375 ...
By KoBioLabs
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Increased expression of genes in a radioresistant cell strain: modulation of hnRNP E2, Hsp90 and SSBP2 genes in γ-irradiated Chinese hamster V79 cells
The methotrexate-resistant cell strain M5, derived from Chinese hamster lung fibroblast cell line V79, provides a good model to study differential gene expression in response to stress. Genes coding for human homologues of myosin phosphatase (AF458589), filamin-like protein (AAA92644), phosphate transporter 1 (AAH199441) and hnRNP E2 (NP_035172.1) were upregulated in M5 as determined by RAP-PCR. ...
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The Impacts of COVID-19 on Scientific Research - and What to Expect Moving Forward
Back in early 2020, amongst the worthy efforts to halt its spread, the COVID-19 pandemic started wreaking havoc on scientific endeavours all over the world. However it did not affect all scientists the same way. While some had their research grind to a halt, others have pivoted or have been redirected to support the new global cause – everything from conducting RT-PCR ...
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Lactobacillus paracasei KBL382 administration attenuates atopic dermatitis by modulating immune response and gut microbiota
ABSTRACT Administration of probiotics has been linked to immune regulation and changes in gut microbiota composition, with effects on atopic dermatitis (AD). In this study, we investigated amelioration of the symptoms of AD using Lactobacillus paracaseiKBL382 isolated from the feces of healthy Koreans. Mice with Dermatophagoides farinaeextract (DFE)-induced AD were fed 1 × 109 CFU ...
By KoBioLabs
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Host Cell Residual DNA: Understanding its Significance and Detection Methods
Host Cell Residual DNA (rcDNA) refers to fragments of DNA derived from host cells that may be present in biological products. These products must not contain foreign substances, particularly host DNA, to avoid immune rejection and potential threats to life safety. Regulatory agencies worldwide have imposed strict limits on the amount of rcDNA, and various pharmacopoeias have outlined several ...
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Genome-Wide Identification Of Chromium Stress-Responsive Micrornas And Their Target Genes In Tobacco (Nicotiana Tabacum) Roots
Tobacco can easily accumulate certain heavy metals in leaves and thus poses a potential threat to human health. To systematically dissect Cr-responsive miRNAs and their targets at the global level, four small RNA libraries were constructed from the roots of Cr-treated (Cr) and Cr-free (control) two contrasting tobacco genotypes,Yunyan2 (Cr-sensitive) and Guiyan1 (Cr-tolerant). Using ...
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Secretin receptor deletion in the subfornical organ attenuates the activation of excitatory neurons under dehydration
School of Biological Sciences, The University of Hong Kong, Hong Kong, China. School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong, China; Gerald Choa Neuroscience Centre, The Chinese University of Hong Kong, Hong Kong, China. School of Life Sciences, Department of Biology, Southern University of Science and Technology, Shenzhen, Guangdong, ...
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hspCas9-RT-PCR Primer Set
Overview Confirm Cas9 mRNA expression With our ready-to-use Cas9 RT-PCR primer set, you can quickly and easily confirm Cas9 expression in cells or in vitro transcription assays. The amplicon for Game 1 is 219 bp and the amplicon for Game 2 2 is 122 bp. These primer sets allow the detection of nuclease Cas9, Nickase Cas9 and double mutant Cas9 at the messenger level. The primer sets are compatible ...
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qPCR for SARS-CoV-2 Detection and Research: Introduction, Principles, and Applications
Introduction to Quantitative Polymerase Chain Reaction A highly delicate technique for the amplification and identification of deoxyribonucleic acid is polymerase chain reaction (PCR). In molecular biology, its conceptual simplification has made it the most utilized method and can, in theory, identify as little as a single segment of DNA. Therefore, for a huge variety of bacterial, fungal, viral, ...
By CD Genomics
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