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Real Time Qpcr Articles & Analysis

4 articles found

Multi-Method Approaches for Collagen Quantification and Analysis

Multi-Method Approaches for Collagen Quantification and Analysis

Western BlotThis method can be used to detect and quantify the relative levels of specific collagen in tissue or cell lysates.Molecular Biology Methods1. Quantitative Real-Time Polymerase Chain Reaction (qPCR)By measuring the mRNA level of the collagen gene, the expression level of collagen can be indirectly inferred. ...

ByMtoZ Biolabs


Multiplex real-time PCR: A guide to application

Multiplex real-time PCR: A guide to application

Multiplex real-time PCR: A guide to application In multiplex real-time PCR (qPCR), scientists amplify two or more target genes in the same reaction, using the same reagent mix. ...

ByAnalytik Jena - an EndressHauser Company


Effect of a lipid-based low pKa anti-pathogenic product (R2) on mitigating PRRSV in feed evaluated by quantitative RT-PCR and pig bioassay

Effect of a lipid-based low pKa anti-pathogenic product (R2) on mitigating PRRSV in feed evaluated by quantitative RT-PCR and pig bioassay

All of the feed homogenates were tested by a PRRSV quantitative real-time RT-PCR (RT-qPCR). Seven groups of feed homogenates from Treatment I (NC)-Id,Treatment 2 (PC)-Id,Treatment 3 (Product)-1 d.Treatment 2 (PC)-4d,Treatment 3 (Product)-4d,Treatment 2 (PC)-7d, and Treatment 3 (Product)-7d were selected for pig bioassay.Twenty-one (10-day-old) ...

ByFeed Energy Company (FEC)


Effects of triclocarban, deet and a mixture of pharmaceuticals and personal care products on fathead minnows (Pimephales promelas)

Effects of triclocarban, deet and a mixture of pharmaceuticals and personal care products on fathead minnows (Pimephales promelas)

We exposed adult fathead minnows (Pimephales promelas) for 48 hours to triclocarban (1.4 µg/L), DEET (0.6 µg/L) or to a PPCP mixture consisting of atenolol (1.5 µg/L), caffeine (0.25 µg/L), diphenhydramine (0.1 µg/L), gemfibrozil (1.5 µg/L), ibuprofen (0.4 µg/L), naproxen (1.6 µg/L), triclosan (2.3 µg/L), progesterone (0.2 µg/L), triclocarban (1.4 µg/L), and DEET (0.6 µg/L). Quantitative ...

ByJohn Wiley & Sons, Ltd.

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