rna interference Articles
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Hnrnp h and hnrnp f complex with fox2 to silence fibroblast growth factor receptor 2 exon iiic
The heterogeneous nuclear ribonucleoprotein H (hnRNP) family of proteins has been shown to activate exon inclusion by binding intronic G triplets. Much less is known, however, about how hnRNP H and hnRNP F silence exons. In this study, we identify hnRNP H and hnRNP F proteins as being novel silencers of fibroblast growth factor receptor 2 exon IIIc. In cells that normally include this exon, we ...
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The KREPA3 zinc finger motifs and OB-fold domain are essential for RNA editing and survival of trypanosoma brucei
Three types of editosomes, each with an identical core containing six related KREPA proteins, catalyze the U insertion and deletion RNA editing of mitochondrial mRNAs in trypanosomes. Repression of expression of one of these, KREPA3 (also known as TbMP42), shows that it is essential for growth and in vivo editing in both procyclic (PF) and bloodstream (BF) life cycle stages of Trypanosoma brucei. ...
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Pituitary adenylate cyclase-activating polypeptide 38-mediated rin activation requires src and contributes to the regulation of hsp27 signaling during neuronal differentiation
Pituitary adenylate cyclase-activating polypeptide 38 (PACAP38) is a potent neuropeptide that acts through G-protein-coupled receptors. While it is well established that PACAP mediates both neurotrophic and neurodevelopmental effects, the signaling cascades that underlie these diverse actions remain incompletely characterized. Here we show that the Ras-related Rin GTP-binding protein, a GTPase ...
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A novel h19 antisense RNA overexpressed in breast cancer contributes to paternal IGF2 expression
The H19/IGFf2 locus belongs to a large imprinted domain located on human chromosome 11p15.5 (homologue to mouse distal chromosome 7). The H19 gene is expressed from the maternal allele, while IGF2 is paternally expressed. Natural antisense transcripts and intergenic transcription have been involved in many aspects of eukaryotic gene expression, including genomic imprinting and RNA interference. ...
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Removal of oxidative dna damage via fen1-dependent long-patch base excision repair in human cell mitochondria
Repair of oxidative DNA damage in mitochondria was thought limited to short-patch base excision repair (SP-BER) replacing a single nucleotide. However, certain oxidative lesions cannot be processed by SP-BER. Here we report that 2-deoxyribonolactone (dL), a major type of oxidized abasic site, inhibits replication by mitochondrial DNA (mtDNA) polymerase and interferes with SP-BER by covalently ...
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Understanding The Applications of Immortalized Cell Lines and Exploring Their Future in Biology Research
In the field of biomedical research, immortalized cell lines have proven to be invaluable tools for a wide range of studies. These cell lines, derived from primary cells, possess the unique ability to undergo continuous division, providing researchers with a sustainable and consistent source of cells for experimentation. Additionally, immortalized cell lines have the advantage of maintaining the ...
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Bioinformatics Analysis of Small RNA Sequencing
Small RNAs are important functional molecules in organisms, which have three main categories: microRNA (miRNA), small interfering RNA (siRNA), and piwi-interacting RNA (piRNA). They are less than 200 nt in length and are often not translated into proteins. Small RNA generally accomplishes RNA interference (RNAi) by forming the core of an RNA-protein complex (RNA-induced silencing complex, RISC). ...
By CD Genomics
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RNA Splicing and How to Target It
What Is RNA Splicing? RNA splicing is a post-transcriptional modification process that occurs in eukaryotic cells. When a gene is transcribed, the initial RNA product is called pre-mRNA, which contains both coding regions (exons) and non-coding regions (introns). RNA splicing removes the introns from the pre-mRNA molecule and joins together the exons to form a mature mRNA molecule that can be ...
By CD Genomics
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CD Genomics Perspective: Sequencing-Based Eukaryote Epigenomics
Unlike prokaryotic cells, eukaryotic cells can regulate gene expression at many different levels. Epigenomic changes are inheritable changes in gene expression and are not caused by changes in DNA sequences. In microbial epigenomics studies, molecular processes influencing epigenomic inheritance have been discovered from microbial eukaryotes such as Neurospora crassa and Schizosaccharomyces ...
By CD Genomics
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Has the future of complement therapeutics arrived?
Silence Therapeutics, a pioneer in the development of novel small interfering RNA (siRNA) therapeutics, and Mallinckrodt plc, a global biopharmaceutical company, announced the submission of a clinical trial application (CTA) for SLN501, a siRNA therapy targeting the complement C3 protein, on March 23, 2022. Silence will receive a $3 million milestone payment from Mallinckrodt as a result of the ...
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CRISPR Screening Technology Brings More Versatility to High-Throughput Sequencing
Introduction to CRISPR Screen Sequencing CRISPR Screen technology is used for high-throughput screening, allowing a large number of gene mutant cells to be created and a mutant cell library to be screened in various external environments. The relationship between phenotype and genotype can be further evaluated using high-throughput sequencing and bioinformatics analysis. Furthermore, ...
By CD Genomics
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Epitranscriptomic Modifications and How to Detect Them
What is epitranscriptomic modification? RNA modifications, not limited to purine or pyrimidine bases, including N6-methyladenosine (m6A), 1-methyladenosine (m1A), 5-methylcytidine (m5C), 5-hydroxymethylcytidine (hm5C), 5-formylcytidine (f5C), 5-carboxycytidine (ca5C), inosine (I), pseudouridine (Ψ), and 2′-O-methylation (Nm). RNA modifications can structurally alter the pairing of ...
By CD Genomics
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The Key Technology of RNA Therapy
It is certain that RNA therapy is no longer a no-man's land for medical innovation. Not only have several products been launched and received good market response, but there are also many pipelines in the research and development side to advance to the middle and late clinical stage, and behind the boom are the technological breakthroughs of scientists. There are three key points in the ...
By BOC Sciences
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