Secondary Antibodies Articles & Analysis
5 articles found
The overall workflow of Far-Western Blot technology is similar to Western Blot, but the detection of target proteins in Western Blot requires antibodies, while antibodies are not needed in Far-Western Blot analysis. ...
But we've never had a set "plan" to find the most suitable antibody. In this issue, the editor shares a 10-step "plan" for antibody selection.Determine the Name of the Target ProteinFirst, you need to be clear about what protein you need to detect in order to select the corresponding antibody. ...
Selection of primary host species When using a conjugated secondary antibody combined with a primary antibody without a conjugate, the species selection of the host animal of the primary antibody is more important. For immunohistochemistry, try to choose the primary antibody of a species different from the sample ...
Direct Enzyme-Linked Immunosorbent Assay (ELISA) (1) This is a commonly used method to measure the content of specific proteins. For collagen, antibodies against its specific types can be used.(2) The collagen in the sample binds with the antibodies fixed on the enzyme-labeled plate, then the detection is done using a secondary ...
The cells were then stained with either a PE Mouse IgG1Isotype control antibody (BioLegend, San Diego, CA, USA) or a PE anti-human TMPRSS2 Antibody (BioLegend, San Diego, CA, USA), followed by a live-dead staining using the LIVE/DEAD™ Fixable Far Red Dead Cell Stain Kit (Thermo Fisher Scientific, Waltham, MA, USA). ...