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Spectrometry Articles & Analysis: This-Year
16 articles found
The workflow typically involves high-resolution mass spectrometry (LC-MS/MS) coupled with sophisticated enrichment strategies. ...
Using specialized tools like antibodies that recognize the unique signature left after ubiquitin digestion (the "di-glycine remnant" or K-GG motif), scientists can selectively isolate ubiquitinated peptides from a cell lysate. Mass Spectrometry (LC-MS/MS): This enriched sample is then analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). ...
Recombinant MGP is typically purified using affinity chromatography and validated by SDS-PAGE, Western blot, and mass spectrometry to confirm integrity and modification status. Research Applications of Recombinant MGP Protein 1. ...
The gold standard for Sterol Lipids Analysis is a combination of chromatography and mass spectrometry (LC-MS/MS or GC-MS/MS). Chromatography (LC or GC): This first step acts like a molecular sorting system, separating the different sterol lipids from each other and from other molecules in the sample. Mass Spectrometry (MS): Once separated, the molecules are sent ...
Molecular Techniques: Advanced techniques like PCR and mass spectrometry enable the detailed analysis of antigens and their structural features, helping elucidate the underlying mechanisms of cross-reactivity. ...
Analytical techniques such as SDS-PAGE, Western blotting, and mass spectrometry are often utilized during this stage. Applications of Recombinant Proteins Recombinant proteins produced in CHO cells have myriad applications across various fields. ...
Modern N-glycan analysis employs sophisticated orthogonal techniques including permethylation analysis, exoglycosidase arrays, and ion mobility-mass spectrometry to decipher isomeric structures that conventional methods cannot distinguish. ...
Advanced methods, such as liquid chromatography-tandem mass spectrometry (LC-MS/MS), are indispensable tools. They allow researchers to: (1) Investigate Endocrine Mechanisms: Advanced analysis techniques help researchers study steroid hormone imbalances associated with various conditions, including cortisol dysregulation, reproductive disorders, and adrenal dysfunction in ...
High-performance liquid chromatography(HPLC) and mass spectrometry (MS) are commonly employed techniques in this analysis. These methods help determine if the drug meets the required purity standards and active concentration. 2. ...
Characterization and Quality Control: A comprehensive characterization of the ADC is crucial. Techniques such as mass spectrometry, analytical HPLC, and functional assays are employed to assess the structure, efficacy, and safety of the ADC. ...
Techniques such as reporter assays and mass spectrometry can be employed to quantitatively assess target degradation and measure downstream biological effects. ...
Analytical Chemistry: Methods like HPLC (High-Performance Liquid Chromatography) and mass spectrometry can be used to analyze secreted products, confirming the functional characteristics of the cells. ...
Metabolomics services typically involve techniques like mass spectrometry (MS) and nuclear magnetic resonance (NMR) to identify and quantify numerous metabolites simultaneously. ...
It offers excellent reproducibility and sensitivity. Mass Spectrometry (MS)-Based Methods: Coupled with HPLC, MS provides highly accurate quantification of NAD/NADH levels and enables the identification of related metabolites in a single analysis. ...
Key Analytical Techniques Liquid Chromatography-Mass Spectrometry (LC-MS): LC-MS is the gold standard for glucosinolate analysis. It provides high sensitivity and specificity, allowing for the accurate identification and quantification of glucosinolates and their metabolites. Gas Chromatography-Mass Spectrometry (GC-MS): GC-MS is particularly useful for analyzing ...
One of the most effective methods is mass spectrometry (MS), often combined with liquid chromatography (LC-MS). This technology enables the detailed identification and quantification of lipids and metabolites from small samples like exosomes, which are typically limited in number. ...