Western Blot Articles & Analysis: This-Year
5 articles found
For protein-level quantification, ELISA and western blotting remain widely used. Q3. What are the challenges of working with recombinant CCR1 protein? ...
Recombinant MGP is typically purified using affinity chromatography and validated by SDS-PAGE, Western blot, and mass spectrometry to confirm integrity and modification status. ...
Additionally, the expression levels of core pluripotency transcription factors, including Oct4, Nanog, and Sox2, are quantified using techniques like quantitative PCR and Western blotting. The presence of these markers confirms that the iPSCs maintain their pluripotent characteristics. ...
Analytical techniques such as SDS-PAGE, Western blotting, and mass spectrometry are often utilized during this stage. ...
Techniques such as ELISA, surface plasmon resonance, and Western blotting are commonly used to evaluate these parameters. 5. Expression and Purification Promising candidates are expressed in a suitable host system. ...