Construction and Culture Protocol of Neural Stem Cells

Because neural stem cells (NSCs) have the potential of self-renewal and multidirectional differentiation, the method of suspended neural bulb culture can be used to obtain and study. After processing the embryonic brain tissue into a single cell, only the cells with the ability of self-renewal can be cloned and proliferated into a suspended nerve ball in the culture medium, and with the progress of subculture, the ability of proliferation and differentiation into a variety of neural progeny cells can be maintained.