Claret Bioscience software

Though there are many ways to handle UMI sequences with reads, we show only one example on this page. The SRSLY UMI software tool will demultiplex samples with bcl2fastq and place UMI sequences in the FASTQ headerline for each fragment. After mapping FASTQ into BAM format, the UMI sequence can then be moved to an auxiliary read tag (such as RX: or BC:) for further analysis steps. We provide SRSLYumi, a simple Python package for running BCL2FASTQ, as well as describing the steps for a manual configuration.