88 Articles found
Creative Proteomics Articles
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What Is Metabolomics?
Metabolism is the assembly of biochemical reactions in living organisms, the material basis for life maintenance of living organisms, and an important basis for the study of life activities. Metabolomics studies the endogenous metabolic activities ...
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Exploring the Intricate Link Between Bile Acids and Disease
Bile acids are synthesized in the liver from cholesterol as a raw material. As a natural "emulsifier", they can promote the dissolution, digestion and absorption of fats, fat-soluble vitamins, etc. Meanwhile, as an important signaling molecule, ...
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Application of Capillary Electrophoresis Technology in Biopharmaceuticals
Capillary Electrophoresis (CE) is an analytical technique used in the development and quality control of protein drugs. From medium optimization, clone screening, formulation stability research and purification process monitoring, to protein ...
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Bile Acid: A Comprehensive Exploration of Function and Metabolism
In the realm of biological sciences, bile acids stand as pivotal molecules, playing a multifaceted role in various physiological processes within organisms. Bile acids are specialized molecules synthesized from cholesterol within the liver. These ...
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Application of Flow Cytometry in the Pharmaceutical Industry
Flow cytometry (FC) is an important tool for analyzing complex pathways and responses of single cells, which can track cell phenotypes and functions in multiple dimensions. It is also increasingly applied in drug development. FC not only can ...
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Cytokines in CAR-T Cell Therapy
What is CAR T-cell therapy? Car-t cell therapy involves genetically engineering T cells isolated from patients or allogeneic donors to express chimeric antigen receptors (CAR) that specifically recognize and kill tumor cells. As a "living" ...
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Understanding the Workflow and Practical Applications of Drug Affinity Responsive Target Stability (DARTS)
Introduction of Drug Affinity Responsive Target Stability (DARTS) Drug target protein identification is an important step in the research and development of new drugs. Drug Affinity Response Target Stability (DARTS) is a technique that can ...
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Understanding Residual DNA Testing and Methods for Detecting Residual DNA
Most biological agents enter the body directly without passing through the gastrointestinal tract, so in addition to biological activity, relevant authorities have very strict requirements on impurities in drugs. Among them, the host cells residual ...
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AMPK Signaling Pathway and Function
AMPK (Adenosine 5'-monophosphate (AMP)-activated protein kinase), as an important kinase regulating energy homeostasis, is one of the central regulators of eukaryotic cell and organism metabolism. It is responsible for regulating cellular ...
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Glycosylation of Fc Fusion Proteins: Insights into its Biological Significance
Fc fusion protein (Fc-Fusion) has a complex and diverse molecular structure, and may contain glycosylation modification sites at amino acid 297 of its Fc fragment and the Fab region of some molecules. Moreover, its biological ligands are ...
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The Principles, Features and Applications of BioID
What is BioID Technique? BioID (proximity-dependent biotin identification) is a proximity dependent labeling technology established by Roux et al in 2015 to study protein interactions. It can also be developed to study the interactions between ...
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Protein Primary Structure Characterization Techniques-Mass Spectrometry
Why characterize protein primary structure The molecular structure of therapeutic proteins is complex and diverse, and their properties vary widely. Therefore, specific analytical methods must be developed for various therapeutic protein molecules. ...
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Inflammation, Immunity and Cancer
Relationship Between Immunity, Inflammation and Cancer Immunity is an act of self-protection of the body. The immune response is divided into specific and non-specific. The antigen and antibody responses are generally referred to as specific immune ...
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Characteristics and Applications of Luminex Multiplex Technology
Luminex multiplex assays is a multi-functional, multi-indicator parallel analysis system that organically integrates color-coded beads, laser technology, applied fluidics, the latest high-speed digital signal processor and computer algorithms ...
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Advantages and Process of Chemical Crosslinking Mass Spectrometry
Chemical cross-linking mass spectrometry (CXMS) is an important tool for resolving protein structures and studying protein interactions. The technique uses a chemical cross-linking agent to link two amino acids in close enough spatial proximity ...
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What are Host Cell Proteins and Its Effect on Biopharmaceutical Development
Host cell proteins (HCPs) are process-related impurities produced by host cells and are typically present at low levels in recombinant biopharmaceutical products. Enzyme-linked immunosorbent assay (ELISA) has traditionally been used to monitor the ...
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Advanced Proximity Labeling Techniques
Proximity labeling techniques represent a cutting-edge approach wherein a decoy protein of interest is fused with an enzyme capable of indiscriminately labeling neighboring proteins. These labeled proteins are referred to as "prey" proteins. ...
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Cytokines: Characteristics, Applications, and Quantitative Analysis
Cytokines represent a class of small protein molecules that exhibit diverse biological activities. These molecules are synthesized and secreted primarily by immune cells (including monocytes, macrophages, T cells, B cells, and NK cells) as well as ...
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Strategies for Comprehensive Glycosylation Characterization of Fc-Fusion Proteins
Fc-Fusion proteins exhibit intricate structural diversity and complex glycosylation modifications, necessitating sophisticated techniques for accurate analysis. This article outlines various methodologies for characterizing Fc-Fusion glycosylation, ...
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Cytokine Involvement in Inflammatory Immune Disorders
Cytokines, small molecules with potent biological activity, are primarily synthesized by immune and non-immune cells (e.g., vascular endothelial cells, epidermal cells, fibroblasts) in response to stimulation. These signaling molecules form ...