Single cell RNA-Seq (scRNA Seq) is a tool that enables simple and robust access to the transcriptomes of thousands of single cells – giving unprecedented insight into tissues at single cell resolution. This offers vital information about heterogeneity and cell-specific dynamics, which is key to understanding many diseases, immunity, development and more.
Single nuclei RNA-Seq (sNuc-Seq) is a novel methodology which uses isolated nuclei instead of whole cells to profile gene expression in cells which are difficult to isolate, as well as archived tissue. Using droplet microfluidic technology this enables users to profile thousands of nuclei at low cost and at high throughput.
Single cell RNA-Seq (scRNA-Seq) is a tool that enables simple and robust access to the transcriptomes of thousands of single cells. With plant protoplast RNA-Seq (ppRNA-Seq), Dolomite Bio has now released a standardised application specific to the requirements of plant cells.
Agarose is a naturally occurring polysaccharide hydrogel, extracted from certain types of red seaweed. It has excellent biocompatibility, thermo-reversible gel behavior, is non-toxic and low-cost, all of which make it a versatile and popular material for life-sciences applications [1].
3D cell cultures, such as organoids and spheroids, have distinct advantages over 2D cultures for diverse cell and tissue types as they more closely mimic the natural environments of the cell. The first step in many 3D cell culture protocols is to seed single or multiple cells into a hydrogel matrix or scaffold.