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can be generated from subsets of these libraries in the range of 400 to 13.000 clones spotted in duplicates. The customer provides us with a list of desired clones/genes/fragments or even his own clones. Clones of the customer may have to undergo sub cloning, in order to be able to express protein on the array. We will then analyse the set and, if wanted, may offer some more/different clones to be placed on the array. We also can provide suitable controls (e.g. house keeping genes) to be included on the array. In agreement with the customer, we can then set up the custom-made Protein Array of the desired size.

The hEXselect protein expression library was derived from the hEX1 library by in silico analysis and re-arraying. Genes which were available in a high clone number were reduced to decrease redundancy. Expressed proteins, which were available only as a single copy were doubled for successive production of high density protein Arrays. The hEXselect protein expression library contains 23.806 clones. Like the hEX1-library, it comprises full-length as well as shorter cDNA clones. All clones have been 5’ tag sequenced and are fully annotated. The size of the low redundancy collection allows a complete spotting in duplicates onto a single Protein Array, which saves processing time and hybridisation probe volume. The majority of the clones represent partial proteins, which may include artificial protein sequences corresponding to the 5’UTR sequences, with one third matching to the human proteome. 

The UniPEx protein expression library consists of 2 Arrays representing clones in 2 different modified vectors. In total, more than 100,000 sequenced clones from different protein expression libraries (human fetal brain, T-cells, lung) were analysed in depth for their coding potential. After in-frame analysis only clones with a confirmed in-frame ORF were selected and redundancy with respect to clones per gene was minimized (< 3 fold). In total, 15,456 UniPEx clones represent 7,390 distinct human proteins.