IHC World products

The IHC-Tek Rabbit LSAB Detection Kit is developed for use with user-supplied Rabbit primary antibodies, facilitating the detection of antigens in tissue sections or cell preparations using light microscopy. This kit is particularly suitable for frozen tissue, paraffin-embedded tissue, freshly prepared lymphocytes, fixed culture cells, and also for whole mount IHC staining. A key feature of this detection system is its employment of a horseradish peroxidase (HRP)-labeled streptavidin and biotinylated secondary antibody system, which is widely recognized in immunochemical staining methodology. The enhanced binding strength of the kit's components offers improved sensitivity and reduced non-specific background staining, leading to a higher signal-to-noise ratio in comparison to the traditional avidin-biotin complex (ABC) method. Notably, when using the kit's Antibody Diluent as the primary antibody dilution buffer, the additional step of serum protein blocking may be omitted.