Jiangsu Bioperfectus Technologies Co., Ltd. products

Viral Nucleic Acid Isolation Kit (Silica-Based Spin Column) from Bioperfectus is specially designed for efficient purification of viral RNA and viral DNA from cell-free samples such as serum, plasma, urine, body fluids and the supernatant of viral infected cell cultures. The sample is lysed by incubation with Lysis Solution. The Lysis Solution inactivates RNases, ensuring protection of viral nucleic acids against degradation. The lysed sample is transferred to a spin column where released viral nucleic acids immediately bind to the silica-based filter in the presence of Binding Buffer. The remaining contaminants are removed during three wash steps using Wash Buffer, whereas pure nucleic acids remain bound to the membrane. Pure viral nucleic acids are released from the spin column filter using Elution Buffer. The purified nucleic acids are ready-for-use in subsequent downstream applications of molecular biology.

This kit uses magnetic beads and buffer system with unique separation function and is used in conjunction with nucleic acid extractor to separate and purify high-quality viral nucleic acids from samples. The specially coated magnetic beads have a strong affinity for the nucleic acid in the sample under certain conditions. When the conditions change, the magnetic beads release the adsorbed nucleic acid, so that the nucleic acid in the purified sample can be quickly extracted. This kit is used for the extraction and purification of viral nucleic acids (DNA and RNA) from samples such as serum, plasma, virus culture fluid, cerebrospinal fluid, urine, swab wash, fecal supernatant, etc. The purified nucleic acid can be used in downstream molecular biology experiment.