MoBiTec GmbH products

Vectors for expression of fusion proteins possessing a (His)6-Tag are commonly used for simple purification of recombinant proteins by metal affinity chromatography (e.g. nickel-nitrilotriacetic acid). The MoBiTec His-Tag antibody is very useful in monitoring (His)6-Tag fusion protein expression and purification and shows a markedly increased sensitivity for (His)6-Tags compared with other commercially available anti-His-Tag antibodies.

Expression vectors constructed to allow synthesis of fusion proteins between glutathione-S-transferase (GST) and proteins encoded by inserted foreign DNA fragments are widely used. Antibodies directed against fusion proteins possessing a GST-Tag are very useful for verifying recombinant protein expression as well as for immunoaffinity purification of such proteins expressed in E. coli. The MoBiTec GST-Tag antibody is optimally suited for monitoring GST-Tag fusion protein expression and purification and shows a markedly increased sensitivity for GST-Tags compared with other commercially available anti GST-Tag antibodies

COVID-19 Research: Antiviral Drug Target ? The COVID-19 pandemic is caused by infection of SARS-CoV-2 or severe acute respiratory syndrome coronavirus 2, a positive-sense single-stranded RNA virus. The RNA dependent RNA polymerase (RdRp) is a key drug target for this deadly virus. The gel-based SARS-CoV-2 RdRp assay can be used for visualization of the RNA molecules synthesized by the RNA polymerase. It can also be used to visualize the enzyme inhibition by RdRp inhibitors. High throughput fluorescence-based assay is also available for screening of RdRp inhibitors in a 384-well or 96-well pate format. The Gel-based SARS-CoV-2 RdRp assay kit includes all the reagents for 20 samples in gel-based assays of SARS-CoV-2 RdRp activity.  It includes 75 µl of 10 x Buffer, 10 µl of 50 x RNA template, 12 µl of 50 x NTPs (ATP, UTP, GTP, CTP), 10 µl of 50 x SARS-CoV-2 RdRp and 130 µl of 5 x Gel loading buffer.

OriCell™ Mesenchymal Stem Cell Osteogenic Differentiation Medium consists of optimized Mesenchymal Stem Cell (MSC) Osteogenic Differentiation Basal Medium, cell culture supplements, and pre-selected fetal bovine serum. This product has been developed for the optimal differentiation of mesenchymal stem cells (MSCs) into osteoblasts.