Automated NGS Library Preparation on CyBi-FeliX - Application Note

1. Introduction

Automation of library preparation is very important to reduce opportunities for error, increase reproducibility, and reduce the amount of hands-on time allowing researchers to generate sequence data from DNA more efficiently. By utilizing the CyBi-FeliX, a benchtop instrument for a variety of automated liquid handling tasks, the HaloPlex Target Enrichment System Kit for NGS library preparation for Ion Torrent™ Sequencing was automated to achieve reliable final prepared libraries with high accuracy for downstream sequencing. This application note describes the successful proof of principle to generate high-quality DNA libraries on CyBi-FeliX for personal genome analysis with the Ion Torrent™ Sequencing System.