Beyond Sequencing CRISPR Gene Editing Assessment and Quality Control Using Next-Generation Sequencing

Sequencing-based genome editing validation can detect whether alleles of a gene have been edited correctly, identify insertion and deletion sites at the whole-genome level, as well as screen and identify monoclonal cell lines. In addition, it is an excellent way to study the off-target effects of CRISPR gene editing. NGS is a massively parallel sequencing strategy that allows for higher throughputs.