CELLINK Vivoink - Bioprinting Protocol - Brochure

This is a suggested procedure, please adjust it according to your experimental needs. Work under aseptic conditions. Protocol aim: The aim of this protocol is to provide instructions for bioprinting of CELLINK Vivoink using the BIO X or BIO X6. It covers steps of pre-print mixing with cells, 3D bioprinting, and post-print processes such as ionic crosslinking. This protocol was optimized for CELLINK Vivoink, undiluted as well as using a 9+1 cell suspension dilution. Changing the dilution parameters in the protocol might change the recommended bioprinting parameters and crosslinking time required. This protocol was optimized for the Pneumatic Printhead and Syringe Pump Printhead on the BIO X6.