Gel-based SARS-CoV-2 RdRp Assay Kit - GS2RPA020KE Brochure

COVID-19 Research: Antiviral Drug Target ? The COVID-19 pandemic is caused by infection of SARS-CoV-2 or severe acute respiratory syndrome coronavirus 2, a positive-sense single-stranded RNA virus. The RNA dependent RNA polymerase (RdRp) is a key drug target for this deadly virus. The gel-based SARS-CoV-2 RdRp assay can be used for visualization of the RNA molecules synthesized by the RNA polymerase. It can also be used to visualize the enzyme inhibition by RdRp inhibitors. High throughput fluorescence-based assay is also available for screening of RdRp inhibitors in a 384-well or 96-well pate format. The Gel-based SARS-CoV-2 RdRp assay kit includes all the reagents for 20 samples in gel-based assays of SARS-CoV-2 RdRp activity.  It includes 75 µl of 10 x Buffer, 10 µl of 50 x RNA template, 12 µl of 50 x NTPs (ATP, UTP, GTP, CTP), 10 µl of 50 x SARS-CoV-2 RdRp and 130 µl of 5 x Gel loading buffer.