Huabio - Model MSH2- ER1803-86 - Rabbit Polyclonal Antibody - Brochure

ER1803-86 was shown to specifically react with MSH2 in wild-type SCC7 cells. No band was observed when MSH2 knockout samples were tested. Wild-type and MSH2 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-MSH2 antibody (ER1803-86, 1/1,000) and Anti-HSP90 antibody (ET1605-56, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.  This substance/mixture is not considered hazardous by the 2012 OSHA Hazard Communication Standard (29 CFR 1910.1200).