expression-system-for-protein-expression Downloads
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pEASY®-Blunt E1 Expression Vector is constructed from pET vector, it utilizes a highly efficient, five-minute blunt cloning strategy to clone PCR product into high-efficient expression vector. The size of control insert is 750 bp, and expressed target protein is about 27 ...
Vectors for expression of fusion proteins possessing a (His)6-Tag are commonly used for simple purification of recombinant proteins by metal affinity chromatography (e.g. nickel-nitrilotriacetic acid). The MoBiTec His-Tag antibody is very useful in monitoring (His)6-Tag fusion protein expression and purification and shows a markedly increased sensitivity for (His)6-Tags compared with other ...
CXCL12 is a ligand for the G protein coupled receptor CXCR4 and induces chemotactic migration in a wide range of CXCR4-expressing cells. CXCL12 protein is expressed in E. coli, refolded and cleaved to yield the native, secreted form of the mature ...
Recombinant HIV-1 p24 protein produced in E.coli Expression ...
Alpha-1-acid glycoprotein (AGP) is a heavily glycosylated protein that is expressed in the liver and secreted into blood. It is a positive acute phase reactant; liver expression and serum concentrations increase as a result of injury, infection or disease. In studies at Life Diagnostics, Inc. we found that serum AGP levels increased approximately 25-fold twenty-four hours after rats were ...
Easily perform advanced transfection protocols with multiple groups of pulses—each with separate parameters, with the BTX AgilePulse PLUS waveform generator. This system is ideal for high efficiency transfection of mammalian cells, including difficult to transfect cell types, such as stem cells and primary cells. It is also suitable for scale up to large volume electroporation for ...
Recombinant Hepatitis C virus Core protein is expressed in E. coli as a monomeric, non-glycosylated polypeptide chain consisting of 115 amino acids with a molecular mass of 13100 Da. The product represents amino acids 1 to 115 of the HCV polyprotein, covering the N-terminal region (“domain I”) of the mature Core protein. It lacks the C-terminal signal peptide, the transmembrane region ...
Recombinant Chikungunya Virus-Like Particles produced in mammalian HEK293 cells. Chikungunya structural proteins are transiently expressed to form a particle comprising E1, E2 and capsid protein, without the non-structural proteins or genome. Resultant particles are totally non-infectious. >90% ...
Product Code: AZ 6267. Source: Baculovirus recombinant protein expressed in insect cells. Presentation. Frozen liquid in PBS buffer, pH 7.4. Preservative: 0.05% sodium azide. Purification Method: The protein is purified by proprietary affinity chromatography method. Purity: Greater than 95% by SDS-PAGE. Distinct band visible at ~50kDa.. Protein Concentration: ≥1 mg/ml (by Bradford assay). ...
Product Specifications: Immobilized Metal Affinity Chromatography (IMAC) purified yeast NADH: nitrate reductase, 5 - 15 U/mg total protein. Modified method of Barbier, GG, RC Joshi, ER Campbell & WH Campbell (2004) Protein Expression and Purification 37: ...
Human Glial Fibrillary Acidic Protein (GFAP) is a 49,749 dalton protein (432 amino acids) expressed by astrocytes of the central nervous system. GFAP is an intermediate filament protein and acts as an intra-cellular structural component of the astrocytic cytoskeleton. During embryonic and fetal life, GFAP is also expressed by radial glial cells of the CNS. Rare mutations of the GFAP gene in ...
ESF 921™ Insect Cell Culture Medium is a complete serum-free, protein-free medium developed for robust cell growth, protein expression and baculovirus vector production for a wide range of insect cells including Sf9, Sf21, Tni, and Drosophila S2. ESF 921 contains LGlutamine and Kolliphor® P188 (Pluronic® F-68). For Research Use Only. Not for use in diagnostic ...
Proteomics is a discipline that analyzes the dynamics of protein components, including expression levels and modification states from a holistic perspective, understands the interactions and connections between proteins, reveals the function of proteins and the laws of cell life, and studies all proteins in cells and their behaviours. ...
ESF AF™ Insect Cell Culture Medium is an Animal Origin-Free formulation and is a complete serum-free, protein-free medium developed for robust cell growth, protein expression and baculovirus vector production for a wide range of insect cells including Sf9, Sf21, Tni, and Drosophila S2. ESF AF medium is comparable to ESF 921 for growth and expression. ESF AF contains L-Glutamine and ...
Expression vectors constructed to allow synthesis of fusion proteins between glutathione-S-transferase (GST) and proteins encoded by inserted foreign DNA fragments are widely used. Antibodies directed against fusion proteins possessing a GST-Tag are very useful for verifying recombinant protein expression as well as for immunoaffinity purification of such proteins expressed in E. coli. The ...
Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20? for 24 months (Avoid repeated freeze / thaw cycles.) Background (Function): 14-3-3 theta (also known as YWHAQ, or 14-3-3 tau) is a member of 14-3-3 proteins which were the first phosphoserine/phosphothreonine-binding proteins to be discovered. 14-3- 3 family members interact with a wide spectrum of proteins and possess diverse ...
Exosomes can affect cell functions by transferring substances such as nucleic acids, proteins, and lipids between cells. For example, they play an important role in the process of antigen presentation in antigen-presenting cells, in the development of tumor cells, and in signal transduction in nerve cells. ...
Chromium Single Cell Gene Expression provides single cell transcriptome 3' gene expression and multiomic capabilities to profile tens of thousands of cells. Explore cellular heterogeneity, novel targets, and biomarkers with combined gene expression, surface protein expression, or CRISPR edits in each ...