primary-antibodie Downloads
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In general the use of Analytik Jena's monoclonal antibodies is possible for all common immunochemical techniques as primary or secondary antibodies. Reactivity of monoclonal antibodies depends on origin of samples and their pre-treatment as well as on reaction conditions. Follow the recommendations for use and possibilities given ...
Our anti-mCherry chicken primary antibody from AvesLabs is manufactured in-house. It detects mCherry, dsRED, RFP (all tested) and tdTOMATO (by similarity) and is purified. It is provided in liquid form. ...
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Bertin Bioreagent distributes primary monoclonal antibodies for various applications, including but not limited to ELISA, Western Blot, Immunofluorescence, Immunocytochemistry, Immunohistochemistry. Our antibodies are mostly used for research areas such as neurodegenerative diseases, epigenetics, and DNA repair. They can also help researchers study transcription factors and molecules related to ...
The Universal HRP-Polymer detects both mouse and rabbit antibodies.This biotin-free technology uses a specific probe to detect mouse primary antibodies and is then followed by a horseradish peroxidase polymer (HRP) that binds to the probe. This innovative HRP-polymerization technology provides increased staining sensitivity for mouse monoclonal antibodies when compared to Envision + and ...
This biotin-free technology uses a specific probe to detect mouse monoclonal primary antibodies which is then followed by an alkaline phosphatase (AP) polymer that binds to the probe. This innovative AP-polymerization technology provides up to 5 to 20 times greater staining sensitivity compared to Envision + and other polymer detection kits. It can be used manually and on automated ...
MACH 2 Mouse HRP-Polymer Detection is a one-step micro-polymer detection system that combines superior workflow and sensitivity for mouse primary antibodies. The horseradish peroxidase (HRP) labeled goat anti-mouse micro-polymer secondary antibody reacts with heavy and light chains on mouse IgG of all subclasses. The innovative micro-polymer technology provides a 3- to 4- fold increase in ...
ICC staining of PRMT1 in D3 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1704-19, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary ...
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Western blot analysis of Cytokeratin 17 on SiHa cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:2,000 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. ...
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Western blot analysis of GARS on Raji cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1610-67, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:40,000 dilution was used for 1 hour at room temperature. ...
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Western blot analysis of HIF-1 beta on human liver tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1609-67, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. ...
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ER1803-86 was shown to specifically react with MSH2 in wild-type SCC7 cells. No band was observed when MSH2 knockout samples were tested. Wild-type and MSH2 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-MSH2 antibody (ER1803-86, 1/1,000) and Anti-HSP90 antibody (ET1605-56, ...
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